Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn

LIU Wenjia; LIU Tianyi; ZHANG Limin; XU Chang; MO Jixian

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LIU W J, LIU T Y, ZHANG L M, et al. Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−11

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LIU W J, LIU T Y, ZHANG L M, et al. Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−11

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Development of microencapsulated rhizosphere growth promoting bacteria and evaluation of the growth promoting effects on corn

1.
College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang　161006, China

Received Date: 2023-12-22
Rev Recd Date: 2024-02-07

Available Online: 2024-03-28

Abstract

Abstract

Objective In order to improve the stability of PGPR in promoting crop growth, microcapsule microbial agents were developed using PGPR composite bacteria as raw materials, and its growth-promoting effects on corn were investigated. Method Isolation of PGPR strains from corn rhizosphere soil and preparation of composite liquid microbial agents. Sodium alginate (SA) and CaCl₂ were used as embedding and crosslinking agents to embed the composite bacterial solution and prepare microcapsule microbial agents. Based on the difficulty level, strain embedding rate, and pre-proliferative and post-proliferative viable bacterial count in the preparation of composite microcapsule microbial agents, the optimal conditions for preparing microcapsule microbial agents were determined. The excellent characteristics of PGPR microcapsules were determined by analyzing the strains growth in PGPR microcapsules under different concentrations of PEG, temperature, pH, storage time, and salt concentration, as well as their effects on corn growth and root morphology. Result This study isolated two PGPR strains with various plant growth-promoting functions. The microcapsule microbial agents made with 1% SA-3% CaCl₂ had good stability, with an embedding rate of 91.27%. After proliferation, the number of live bacteria in the microcapsule reached 8.73 × 10⁹ cfu·g⁻¹, with a proliferation rate of 7.79 times. Under different concentrations of PEG, temperature, pH, storage time, and salt concentration, the concentration of microencapsulated bacterial solution was higher than that of liquid bacterial solution. PGPR microcapsule microbial agents had significant promoting effects on corn growth. Applying microcapsule microbial agents increased corn plant height, seedling dry weight, and root dry weight by 91.83%, 81.82%, and 29.57%, respectively. At the same time, microencapsulated microbial agents significantly increased the total root length, root surface area, average root diameter, root volume, and root tip number of corn. Conclusion This study determined a preparation method for PGPR microcapsule microbial agents. The application of the PGPR microcapsule agents significantly promoted corn growth, improved corn root morphology, and had better growth-promoting effects on corn than liquid microbial agents.
- Corn,
- Rhizosphere growth promoting bacteria,
- Promoting growth effects,
- Microcapsule microbial agents

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References(29)

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