Isolation and identification of a novel duck adenovirus B2 strain with insertion and deletion mutation characteristics

GUO Bolun; LI Yaru; ZHENG Xin; JIANG Dandan; ZENG Li; CHEN Shaoying; MA Yanmei; CHEN Shilong

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GUO B L, LI Y R, ZHENG X, et al. Isolation and identification of a novel duck adenovirus B2 strain with insertion and deletion mutation characteristics [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−6

Citation:

GUO B L, LI Y R, ZHENG X, et al. Isolation and identification of a novel duck adenovirus B2 strain with insertion and deletion mutation characteristics [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−6

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Isolation and identification of a novel duck adenovirus B2 strain with insertion and deletion mutation characteristics

GUO Bolun^{1, 2
,},
LI Yaru^{1, 2},
ZHENG Xin^{1, 2},
JIANG Dandan^{2, 3},
ZENG Li^{2, 3},
CHEN Shaoying^{2, 3},
MA Yanmei^{1
,
,},
CHEN Shilong^{2, 3
,
,}

1.
College of Animal Sciences, Fujian Agricultural and Forestry University/Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, Fuzhou, Fujian　350002, China
2.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian　350013, China
3.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350003, China

Received Date: 2024-01-01
Rev Recd Date: 2024-02-01

Available Online: 2024-03-28

Abstract

Abstract

: Objective In order to isolate duck adenovirus B2 (DAdV B2) strain from "Pale liver disease" and provide a reference for the DAdV B2 epidemiological study in Fujian Province. Methods The samples were detected by PCR, and the virus isolation was carried out and identified by the whole genome sequencing and ducklings challenge. Result A DAdV B2 strain, named DAdV B2/BG48, was successfully isolated from duckling pale liver disease. DAdV B2/BG48 infected LMH cells became large and round, and finally died and disintegrated. MDEF cells with with DAdV B2/BG48 inoculation became round and clustered together with intercellular space appeared. The results of next generation sequencing showed that BG48 genome had a 3 bp insertion in the pX gene region, a 33 bp insertion in the ORF19B gene domain, and 42 bp deletion at the junction of ORF64 and ORF67. There was no truncated mutation in the ORF67 gene. Other coding genes had no characteristic differences as compared to previously identified strains such as CH-GD-12-2014, BG27 and BG18. Duckling challenge experiments showed that the morbidity and mortality of BG48 to 2-day-old Muscovy ducks were 50% and 0%, respectively, and the clinical symptoms and pathological changes of diseased ducklings were similar to those of naturally infected ducks. Conclusion A novel DAdV B2 strain named BG48 was successfully isolated and identified from a case of "Pale liver disease" in Muscovy duck. BG48 has the characteristics of multi-site insertion and deletion, suggesting that DAdV B2 strain was prone to mutation with a complex clinical epidemic. These results provide a reference for the molecular epidemiological investigation and the genetic evolution of DAdV B2.
- White liver disease,
- Duck adenovirus B2,
- Isolation and identification,
- Insertion and deletion,
- ORF67

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References(17)

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