A new method—LiCl for extracting bacterium plasmid DNA

PAN Jian-jing; ZHANG Jun-cheng; LU Yi-ping; ZHANG Hang-ying; ZHUANG Wei-jian

Volume 22 Issue 4

Mar. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2007 > 22(4): 469-470

PAN Jian-jing, ZHANG Jun-cheng, LU Yi-ping, ZHANG Hang-ying, ZHUANG Wei-jian. A new method—LiCl for extracting bacterium plasmid DNA[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 469-470.

Citation:

PAN Jian-jing, ZHANG Jun-cheng, LU Yi-ping, ZHANG Hang-ying, ZHUANG Wei-jian. A new method—LiCl for extracting bacterium plasmid DNA[J]. Fujian Journal of Agricultural Sciences, 2007, 22(4): 469-470.

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A new method—LiCl for extracting bacterium plasmid DNA

1.
College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Sanming University, Sanming, Fujian 365000, China

Received Date: 2007-02-27
Rev Recd Date: 2007-12-12
Publish Date: 2007-12-15

Abstract

Abstract

A new method to extract small amount of plasmid DNA with LiCl was developed.The results showed that the quality of plasmid DNA extracted by using this method was basically identical to that of the conventional alkalinelysis.The method satisfied the need for basic molecular biology experiments,and the procedure was simplified shortening the extracting time by 24 min.
- Plasmid DNA,
- LiCl

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References(2)

References

[1]	F.奥斯伯,R.布伦特,R.E.金斯顿,等.精编分子生物学实验指南[M].北京:科学出版社,1998:17-19.
[2]	姚红艳,赵双宜,夏光敏.改良尿素-氯化锂方法提取成熟小麦种子总RNA[J].中国生物工程杂志,2003,23(4):86-88.