PCR detection and characterization of a phenol-degrading bacteria(<i>Alcaligenes faecalis</i> BC2001)

LIN Zhi-min; Song Ya-na; YAO Mei-bin; CHEN Bin; ZHENG Wei-wen

Volume 22 Issue 1

Apr. 2012

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LIN Zhi-min, Song Ya-na, YAO Mei-bin, CHEN Bin, ZHENG Wei-wen. PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 50-53.

Citation:

LIN Zhi-min, Song Ya-na, YAO Mei-bin, CHEN Bin, ZHENG Wei-wen. PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)[J]. Fujian Journal of Agricultural Sciences, 2007, 22(1): 50-53.

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PCR detection and characterization of a phenol-degrading bacteria(Alcaligenes faecalis BC2001)

1.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Environmental Engineering College, Northeast Agricultural University, Harbin, Heilongjiang 150030, China

Received Date: 2006-11-03
Rev Recd Date: 2006-12-11
Publish Date: 2007-03-15

Abstract

Abstract

A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.A 684 bp fragment was amplified from Alcaligenes faecalis BC2001’s total DNA.After sequencing and alignment by Blast,the sequence share 97% homology with phenol-degrading Proteobacterium L-18 strain(accession number:AY346142).Another phenol-degrading experiment showed that pH 6 was optimum for the growth of BC2001.It exemplified the BC2001 strain showing its phenol-degrading ability at 300 mg·L-1 of phenol concentration.
- phenol hydroxylase gene,
- PCR detection,
- Alcaligenes faecalis BC2001,
- degradation

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References(6)

References

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