Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Citation:
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Citation:
HUANG Su-fang, CHE Jian-mei, LIU Bo, SHI Huai, SU Ming-xing, CHEN Zheng. Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX[J]. Fujian Journal of Agricultural Sciences, 2011, 26(4): 528-532.
Different extraction methods were compared in extracting of active substances from metabolites of Brevibacillus brevis FJAT-0809-GLX.It macroporous tesin method provided the best effect in extraction with 1.593 gL-1.Antibacterial activities of the extract was higher in it by macroporous resin method than it by the chloroform.The optimal procedures applied for extraction of active substances were: centrifuging the fermentation broth of FJAT-0809-GLX at 3600 rpm for 30 min,mixing the supernatant with 40 gL-1 macroporous resin(AmberliteXAD16)and then vortexing for 4h at 160 rpm,28℃;filling the column with the mixture and eluting it with acetone in the ratio of 3 times in given volum of extraction.The eluent was collected and concentrated rotary evaporation at 40℃.
DownLoad: