Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks

WAN Chun-he; SHI Shao-hua; CHENG Long-fei; CHEN Hong-mei; FU Guang-hua; PENG Chun-xiang; LIN Fang; LIN Jian-sheng; HUANG Yu

Volume 26 Issue 1

Nov. 2011

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Article Navigation > Fujian Journal of Agricultural Sciences > 2011 > 26(1): 10-12

WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.

Citation:

WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.

Citation:

WAN Chun-he, SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, PENG Chun-xiang, LIN Fang, LIN Jian-sheng, HUANG Yu. Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2011, 26(1): 10-12.

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Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2011-01-05
Rev Recd Date: 2011-01-26
Publish Date: 2011-02-15

Abstract

Abstract

A pair of primers based on NS5 gene sequences of different strains of genus Flavivirus was designed for amplification of 470 bp fragments.No positive target fragment was available from H9 subtype influenza virus,Muscovy duck reovirus,duck plague,duck hepatitis virus(DHV-Ⅰ) or duck-origin avian paramyxovirus typeⅠ.The detection limit reached 20 pg under the optimized conditions.The results showed that the RT-PCR was sensitive,specific and applicable for rapid laboratory diagnosis and epidemiologic surveillance.
- ducks hemorrgagic ovaritis,
- Flavivirus,
- RT-PCR

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References(2)

References

[1]	万春和,施少华,程龙飞,等.朗德鹅圆环病毒全基因组序列测定和遗传演化分析[J].中国动物传染病学报,2010,18(4):6-12.
[2]	JACKSON G.MCNICHLOS R,FOX G,et al.Toward universal Flavivirus identification by mass cataloging[J].Journal of Molecular Diagnostics,2008,10 (2):135-141.