Identification and Expression Analysis of TIFY Gene Family in <i>Isodon rubescens</i>

LIAN Conglong; LAN Jinxu; YANG Jingfan; LI Jingjing; YANG Hao; CHEN Suiqing

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LIAN C L, LAN J X, YANG J F, et al. Identification and Expression Analysis of TIFY Gene Family in Isodon rubescens [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−12

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LIAN C L, LAN J X, YANG J F, et al. Identification and Expression Analysis of TIFY Gene Family in Isodon rubescens [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−12

LIAN C L, LAN J X, YANG J F, et al. Identification and Expression Analysis of TIFY Gene Family in Isodon rubescens [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−12

Citation:

LIAN C L, LAN J X, YANG J F, et al. Identification and Expression Analysis of TIFY Gene Family in Isodon rubescens [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−12

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Identification and Expression Analysis of TIFY Gene Family in Isodon rubescens

1.
School of Pharmacy, Henan Key Laboratory of Chinese Medicine Resources and Chemistry/Henan University of Chinese Medicine, Zhengzhou, Henan　450046, China

Received Date: 2023-10-11
Rev Recd Date: 2023-12-02

Available Online: 2024-03-28

Abstract

Abstract

Objective TIFY proteins are key regulators of JA signaling pathway, which also play significant regulatory roles in plant growth and development, abiotic stress, and secondary metabolites accumulation. Study the TIFY genes of Isodon rubescens can provide a theoretical basis for the improvement breeding of stress resistance and the synthesis of secondary metabolites in I. rubescens. Method In this study, the TIFY gene family was identified and analyzed by bioinformatics methods based on the full-length transcriptome database of I. rubescens, and the expression characteristics of the TIFY gene family in different tissues were analyzed by RT-qPCR. Result (1) A total of 12 TIFY genes were identified in the full-length transcriptome data of I. rubescens. (2) Physicochemical properties analysis showed that the amino acid length of IrTIFYs ranged from 124 aa to 378 aa. The molecular weight ranges from 13924.89 to 39692.38 Da. The isoelectric points were rang from 5.05 to 9.69; All the proteins were belong to stable proteins except IrTIFY10. The subcellular location analysis showed that all IrTIFY proteins were located in the nucleus, and all TIFYs are belong to hydrophilic proteins without signal peptides. (3) The structural analysis showed that none of the IrTIFY proteins had a transmembrane structure, and random curling was the most abundant structure type in the secondary structure. All proteins contains multiple phosphorylation sites. (4) Codon preference analysis showed that the codon preference of IrTIFY gene family was weak, and the codon ending with A or U was slightly inclined to be used. (5) The cis-elements analysis of IrTIFYs promoter showed that there were many light responsive cis-elements, hormone responsive cis-elements, and stress responsive cis-elements in the TIFY family. However, there were differences in the numbers and types of cis-elements among different IrTIFY members.（6）The phylogenetic tree analysis showed that 12 IrTIFYs were divided into four subfamilies: PPD (IrTIFY2), ZML (IrTIFY3/8/10), TIFY (IrTIFY7/12) and JAZ (IrTIFY1/4/5/6/9/11). and IrTIFYs had the closest genetic relationship with Salvia miltiorrhiza, which also belong to the labiaceae family. (7) RT-qPCR analysis showed that the expression levels of 12 TIFYs exhibited same trend with leaf ＞ stem ＞ root of different tissues, and most of the members showed significant differences between different tissues. Conclusion Based on the above results, it is speculated that IrTIFYs play an important regulatory role in the growth and development of I. rubescens, and may be involved in regulating the biosynthesis of secondary metabolites of I. rubescens, which lays a foundation and provides ideas for further research on the function of TIFY gene family in I. rubescens.
- Isodon rubescens,
- TIFY gene family,
- Bioinformatics analysis,
- Expression analysis

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References(29)

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