Gene Cloning and Functional Analysis of <i>MebZIP2</i> in Cassava

CHEN Ganlu; YAN Yan; MENG Xianwei; FU Lili; QIU Xianjin; DING Zehong; HU Wei

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CHEN G L, YAN Y, MENG X W, et al. Gene Cloning and Functional Analysis of MebZIP2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−10

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CHEN G L, YAN Y, MENG X W, et al. Gene Cloning and Functional Analysis of MebZIP2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−10

CHEN G L, YAN Y, MENG X W, et al. Gene Cloning and Functional Analysis of MebZIP2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−10

Citation:

CHEN G L, YAN Y, MENG X W, et al. Gene Cloning and Functional Analysis of MebZIP2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−10

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Gene Cloning and Functional Analysis of MebZIP2 in Cassava

CHEN Ganlu^{1, 2
,},
YAN Yan²,
MENG Xianwei²,
FU Lili²,
QIU Xianjin^{1
,
,},
DING Zehong^{2, 3
,
,},
HU Wei^{2, 3}

1.
College of Agriculture, Yangtze University, Jingzhou, Hubei　434025, China
2.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, Hainan　571101, China
3.
Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan　572025, China

Received Date: 2023-10-12
Rev Recd Date: 2023-11-15

Available Online: 2024-03-28

Abstract

Abstract

: Objective bZIP transcription factors play an important role in plant growth and development, starch synthesis, and abiotic stress. Cloning of cassava MebZIP2 and analysis of its subcellular localization, gene expression, and yeast one-hybrid can provide useful references to further study the function of MebZIP2. Method The coding sequence (CDS) of MebZIP2 was amplified from cassava SC205 and then used to perform bioinformatics analysis. A pNC-Green-SubN fusion expression vector was constructed and transferred into tobacco epidermal cells by Agrobacterium-mediated method, and fluorescence signals were observed to determine subcellular localization of the MebZIP2 protein. The expression profiles of MebZIP2 were analyzed in different tissues and at different development stages of cassava storage root. The interactions between MebZIP2 and the promoters of starch synthesis genes were studied by yeast one-hybrid. Result The CDS sequence of MebZIP2 was 465 bp in length and encoded 154 amino acids. The molecular weight was 17891.35 Da, the theoretical isoelectric point was 5.23, and MebZIP2 was an unstable hydrophilic protein. MebZIP2 contained a bZIP-conserved domain and showed the highest sequence similarity (74.22%) to a bZIP protein from the Hevea brasiliensis. The promoter region of MebZIP2 contained light-responsive elements, hormone (such as gibberellin, salicylic acid, and jasmonic acid) responsive elements, as well as endosperm expression and stress-responsive elements. MebZIP2 protein was localized both in the cell membrane and nucleus. MebZIP2 showed high expression levels in root apical meristems, fibrous roots, stems, and at early developmental stages of storage roots. MebZIP2 was co-expressed with several starch synthesis genes such as MeAPL5a, MeGBSS1, and MeISA1, and MebZIP2 could interact with their promoters. Conclusion MebZIP2 belonged to the bZIP gene family, and its expression showed tissue-specificity and root developmental stage-specificity. MebZIP2 protein could interact with the promoters of starch synthesis genes (MeAPL5a, MeGBSS1 and MeISA1), and it might be involved in storage root development and starch synthesis in cassava.
- Cassava,
- bZIP transcription factor,
- MebZIP2,
- gene cloning,
- functional analysis

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References(40)

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