Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks

ZHANG Rui; LIU Rongchang; CHEN Changfu; HUAN Yu; CHENG Longfei; FU Guanghua; SHI Shaohua; CHEN Hongmei; WANG Chunhe; FU Qiuling

doi:10.19303/j.issn.1008-0384.2021.03.009

Volume 36 Issue 3

Mar. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(3): 312-318

ZHANG R, LIU R C, CHEN C F, et al. Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks [J]. Fujian Journal of Agricultural Sciences，2021，36（3）：312−318 doi: 10.19303/j.issn.1008-0384.2021.03.009

Citation:

ZHANG R, LIU R C, CHEN C F, et al. Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks [J]. Fujian Journal of Agricultural Sciences，2021，36（3）：312−318 doi: 10.19303/j.issn.1008-0384.2021.03.009

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Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks

doi: 10.19303/j.issn.1008-0384.2021.03.009

1.
College of Animal Sciences (College of Bee Sciences), Fujian Agriculture and Forestry University, Fuzhou, Fujian 　350002, China
2.
Institute of Animal Husbandry and Veterinary Medicine/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian 　350001, China
3.
Center for Animal Disease Control and Prevention, Longyan, Fujian　364000, China

Received Date: 2020-11-19
Rev Recd Date: 2020-12-31

Available Online: 2021-02-08

Publish Date: 2021-03-31

Abstract

Abstract

Objective The pathogen causing a viral disease with a 65% incident rate, 90% mortality, and massive skin hemorrhage on the birds at a mule duck farm in Fuzhou, Fujian in August 2020 was isolated with its pathogenic characteristics identified. Method The heart, liver, spleen, pancreas, kidney, skin, and other tissues of the diseased ducks were collected for the pathogen detection including virus isolation and identification, gene sequencing, and histopathological examinations. Result PCR showed positive results only on the collected tissues from the plague virus-infected ducks. Subsequent virus isolation and identification on those positive specimens were performed, and the virus code-named as FJ2020176. The UL2 gene of the strain was sequenced to show a 97.8-99.9% nucleotide homology with the virulent strain CHV, CV, and 2085. Compared to the vaccine strain VAC, the attenuated strain 1, and the attenuated strain 2, FJ2020176 had a 528bp nucleotide insertion in its UL2 gene confirming it to be a virulent strain of duck plague virus. The histopathological examination under a microscope showed the following observations in the infected ducks: necrosis and hemorrhage on the focal liver, decreased white pulp on the spleen tissue, necrotic and exfoliated lymphocytes, hemorrhage and blood stasis on the kidney, necrosis on the lymph nodes, and hemorrhage on the bursa of Fabricius, in addition to the exterior skin hemorrhage. Conclusion The virulent FJ2020176 was isolated from the diseased ducks with massive skin hemorrhage and severe infection and mortality rates. The bleeding on the skin was deemed a significant symptom for clinical diagnosis of the duck plague cases.
- mule duck,
- duck plague virus,
- isolation and identification,
- histopathology

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References(16)

References

[1]	苏敬良, 黄瑜, 胡薛英. 鸭病学[M]. 北京: 中国农业大学出版社, 2016: 63-80.
[2]	PLAGEMANN P G. Complexity of the single linear neutralization epitope of the mouse Arterivirus lactate dehydrogenase-elevating virus [J]. Virology, 2001, 290(1): 11−20. doi: 10.1006/viro.2001.1139
[3]	LIU T, CHENG A C, WANG M S, et al. RNA-seq comparative analysis of Peking ducks spleen gene expression 24 h post-infected with duck plague virulent or attenuated virus [J]. Veterinary Research, 2017, 48(1): 1−14. doi: 10.1186/s13567-016-0406-1
[4]	李小伦. 鸭瘟病毒感染的诊断与病毒分离鉴定 [J]. 中国动物保健, 2018, 20(1):18−19. doi: 10.3969/j.issn.1008-4754.2018.01.006 LI X L. Duck plague virus infection diagnosis and virus isolation and identification [J]. China Animal Health, 2018, 20(1): 18−19.(in Chinese) doi: 10.3969/j.issn.1008-4754.2018.01.006
[5]	黄引贤, 欧守杼, 邝荣禄, 等. 鸭瘟病毒的研究 [J]. 华南农学院学报, 1980, 1(1):21−36. HUANG Y X, OU S Z, KUANG R L, et al. Research on duck plague virus [J]. Journal of South China Agricultural College, 1980, 1(1): 21−36.(in Chinese)
[6]	姜甜甜, 张大丙. 鸭瘟强毒与疫苗毒的囊膜糖蛋白序列比较 [J]. 中国兽医杂志, 2013, 49(2):3−7. doi: 10.3969/j.issn.0529-6005.2013.02.001 JIANG T T, ZHANG D B. Comparison of the glycoprotein sequences between virulent and attenuated strains of Anatid herpesvirus 1 [J]. Chinese Journal of Veterinary Medicine, 2013, 49(2): 3−7.(in Chinese) doi: 10.3969/j.issn.0529-6005.2013.02.001
[7]	刘荣昌, 黄瑜, 卢荣辉, 等. 鸭瘟病毒的分离鉴定及其UL2、TK 基因序列分析 [J]. 福建农业学报, 2016, 31(12):1257−1261. LIU R C, HUANG Y, LU R H, et al. Isolation and identification of duck plague virus and sequence analysis on its UL2 and TK genes [J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1257−1261.(in Chinese)
[8]	傅光华, 陈翠腾, 刘荣昌, 等. 鸭瘟病毒新近分离毒株部分基因变异分析 [J]. 畜牧兽医学报, 2018, 49(10):2215−2222. doi: 10.11843/j.issn.0366-6964.2018.10.017 FU G H, CHEN C T, LIU R C, et al. Genetic variability of partial genes of duck plaque viruses currently isolated in ducks [J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(10): 2215−2222.(in Chinese) doi: 10.11843/j.issn.0366-6964.2018.10.017
[9]	LIU R C, CHEN C T, HUANG Y, et al. Microbiological identification and analysis of waterfowl livers collected from backyard farms in Southern China [J]. The Journal of Veterinary Medical Science, 2018, 80(4): 667−671. doi: 10.1292/jvms.17-0452
[10]	张红丽, 黄靖, 刘霞, 等. 鸭瘟病毒ZJ2016强毒株部分基因序列的测定与分析 [J]. 浙江农业学报, 2020, 32(2):226−233. ZHANG H L, HUANG J, LIU X, et al. Sequencing and analysis of several genes of a virulent duck enteritis virus strain ZJ2016 [J]. Acta Agriculturae Zhejiangensis, 2020, 32(2): 226−233.(in Chinese)
[11]	祖立闯, 曹文学, 吴春霞. 一例鸭瘟与鸭疫里默氏杆菌混合感染的诊断报告 [J]. 中国动物保健, 2020, 22(9):78−79. doi: 10.3969/j.issn.1008-4754.2020.09.062 ZU L C, CAO W X, WU C X. Diagnostic report of a mixed infection of duck plague and Riemerella anatipestifer [J]. China Animal Health, 2020, 22(9): 78−79.(in Chinese) doi: 10.3969/j.issn.1008-4754.2020.09.062
[12]	田彬, 汪铭书, 程安春. 鸭瘟病毒致病性研究进展 [J]. 中国家禽, 2018, 40(12):38−42. TIAN B, WANG M S, CHENG A C. Progress in pathogenicity of duck plague virus [J]. China Poultry, 2018, 40(12): 38−42.(in Chinese)
[13]	WANG J C, HÖPER D, BEER M, et al. Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains [J]. Virus Research, 2011, 160(1/2): 316−325.
[14]	YUN T, HUA J G, YE W C, et al. Comparative proteomic analysis revealed complex responses to classical/novel duck reovirus infections in the spleen tissue of Cairna moschata [J]. Journal of Proteomics, 2019, 193: 162−172. doi: 10.1016/j.jprot.2018.10.006
[15]	MAMTA P, SHAILESH K P, SYED A A, et al. Pathology of wild strain (DP/As-Km/0019) of duck plague virus infection revived in ducklings [J]. Journal of Animal Research, 2019, 9(6): 831−835.
[16]	刘忠伟, 杨颖, 江楠, 等. 鸭瘟强毒人工感染雏鸭的显微和超微病理学变化 [J]. 江苏农业科学, 2017, 45(16):140−147. LIU Z W, YANG Y, JIANG N, et al. Microscopic and ultrastructural changes of ducklings artificially infected withduck plague virus [J]. Jiangsu Agricultural Sciences, 2017, 45(16): 140−147.(in Chinese)