• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

不同茶季‘金牡丹’五大类茶主要品质成分差异分析

Chemical Differentiations of Spring, Summer, and Autumn 'Jinmudan' Teas

  • 摘要:
    目的 揭示‘‘‘金牡丹’茶的主要品质特征及其春、夏、秋3季中不同茶类(红茶、乌龙茶、白茶、黄茶及绿茶)的主要品质成分差异情况,探究不同茶季金牡丹茶主要特征代谢物间的关联特性以及可用于区分不同茶季的主要代谢物。
    方法 以不同茶季的金牡丹鲜叶制成的红茶、乌龙茶、黄茶、白茶及绿茶为供试材料,基于生化测定、高效液相色谱法以及AQC(6-aminoquinolyl-N-hydroxysuccinimidyl carbamate)衍生液质联用法对供试茶样的生化成分进行测定分析。
    结果 金牡丹夏茶的茶多酚和儿茶素含量高于其他茶季。而春茶中氨基酸含量最高,夏茶最低。金牡丹茶的儿茶素组成以EGC、EGCG、ECG及GC为主。相关性分析表明,不同茶季中特征性代谢物间显著相关。基于OPLS-DA判别模型,在不同茶季的金牡丹红茶、乌龙茶、白茶、黄茶及绿茶中分别筛选出12、15、15、17、17种差异代谢产物,这些代谢物可作为区分春、夏、秋茶的潜在标志。
    结论 本研究明确了金牡丹茶在不同茶类和茶季中的滋味品质化学特征,揭示其不同茶季的主要特征代谢物的变化规律,为提升金牡丹鲜叶原料的利用率及优化金牡丹茶品质提供科学依据。

     

    Abstract:
    Objective Differentiations in the main chemicals related to quality of spring, summer, and autumn Jinmudan teas were analyzed to examine the correlations between metabolites and characteristics of the teas.
    Methods Black, oolong, yellow, white, and green Jinmudan teas made of fresh leaves plucked separately in three seasons were analyzed for biochemical composition using the conventional methods, high performance liquid chromatography, and AQC derivative liquid chromatography tandem triple quadrupole mass spectrometry.
    Results The contents of tea polyphenols and catechins in the Jinmudan summer tea were significantly higher than those in the others, whereas that of amino acids highest in the spring tea and lowest in the summer tea. The catechins in the Jinmudan teas were mainly EGC, EGCG, ECG, and GC. Significant correlation between characteristic metabolites in different tea seasons. As analyzed by the OPLS-DA discriminant model, 12 differential metabolites came from the black tea, 15 from the oolong tea, 15 from the white tea, 17 from the yellow tea, and 17 from the green tea. These metabolites were distinctive enough to serve as markers to separate these teas.
    Conclusion The chemical differences among the varieties of spring, summer, and autumn Jinmudan teas underscored a close relationship between the chemistry and quality characteristics of the teas.

     

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