• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

金线兰茎腐病拮抗木霉菌鉴定及抑菌和促生效果评价

Identification and Evaluation of Stem Rot Antagonistic and Growth promotion Effects of Trichoderma on Anoectochilus roxburghii

  • 摘要:
      目的  分离具有金线兰茎腐病拮抗作用的木霉菌,为生防菌的开发提供理论基础。
      方法  以金线兰仿野生种植植株为材料,利用组织分离法分离木霉菌,利用形态特征与ITS和rpb2序列同源性分析鉴定其分类,利用平板对峙法鉴定其抗茎腐病能力,并对不同木霉菌的促生长作用进行评价。
      结果  利用组织分离法分离3株木霉菌A21B-1、A21B-2和A21E。经鉴定,3株木霉株分别为哈茨木霉拟康宁木霉及Trichoderma longifialidicum。对峙生长表明,3种木霉菌株均对茎腐病病原菌尖孢镰刀菌ASP01表现较强的抑制作用,其抑制率分别达75.29%、73.55%和 60.02%。室内防效结果表明,A21B-1菌株对茎腐病有较强的抑制作用,接种15 d后病情抑制率达91.9%,可作为该病的生物防治候选菌株。促进生长试验表明,种植6个月后,施用3个木霉菌的金线兰植株的单株重、株高、茎粗、叶面积及SPAD值较对照均显著提高,其中A21B-2与A21E处理的植株单株重比对照分别提高了58.6%与58.9%,叶面积分别提高66.8%与59.7%,可作为金线兰促进生长的候选菌株。同时,施用木霉菌可有效提高金线兰多糖及金线莲苷的含量,其中A21B-2菌株效果最佳,其多糖及金线莲苷含量均较对照提高89.6%与11.8%,可作为促进金线兰药用成分积累的候选菌株。
      结论  3种不同类型的木霉菌在金线兰对抗茎腐病、促进生长及提高多糖含量方面有显著作用。

     

    Abstract:
      Objective  Isolation of stem rot antagonistic Trichoderma from Anoectochilus roxburghii, providing theoretical basis for the development of biocontrol fungi. in A. roxburghii.
      Method  Using wild cultivated A.roxburghiias as materials, Trichoderma strains were isolated using tissue isolation method. Morphological characteristics and homology analysis with ITS and rpb2 sequences were conducted for strains classification. Plate confrontation method were used for evaluating different Trichoderma strains resistance to stem rot ability, and the growth promoting effects of different Trichoderma strains were also processed.Resulsts3 Trichodermas trains as A21B-1, A21B-2 and A21E were isolated from the A. roxburghii via tissue isolation method. Combined with morphological characterization and ITS and rpb2 sequences homology identification, 3 Trichoderma strains were identified as T. rugulosum, T. koningiopsis and T. longifialidicum, respectively. Confrontation cultured showed that the 3 Trichoderma strains showed strong inhibitory effects on stem rot pathogen Fusarium oxysporum f. sp. opponiarum ASP01, and their inhibition rates reached 75.29%, 73.55% and 60.02%, respectively. The indoor control results showed that A21B-1 strain had a strong inhibitory effect on stem rot, after 15 d inoculation, the disease inhibition rate reached 91.9%, which could be used as a candidate strain for stem rot biological control fungi. The growth promotion experiments showed that Trichoderma strains significantly increased individual plant weight, height, stem diameter, leaf area, and SPAD value in A. roxburghii after 6 months grown. Among the 3 Trichoderma strains, plants innoculated with A21B-2 and A21E showed significant growth promotion effects with individual plant weight increased by 58.6% and 58.9%, leaf area increased by 66.8% and 59.7% compared to the controls, respectively. They could be used as candidate strains for growth promoting in A. roxburghii. At the same time, the application of Trichoderma strains effectively increased the content of polysaccharides and kinsenoside in A. roxburghii, A21B-2 strain showed the best effects, which content of polysaccharides and kinsenoside increased by 89.6% and 11.8% compared to the controls, could be used a candidate strain for promoting the accumulation of medicinal components in A. roxburghii.
      Conclusion  3 different strains of Trichoderma have significant effects on inhibiting stem rot disease, promoting growth, and increasing polysaccharide content in A. roxburghii.

     

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