• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

不同R/FR比值对低温条件下茶树光合荧光特性及PIFs的影响

Effects of Different R/FR Ratios on Photosynthetic Fluorescence Characteristics and PIFs of Tea Plants under Low Temperature Conditions

  • 摘要:
    目的 探究低温条件下茶树在不同红光与远红光比值(R/FR)光环境中的生理与分子响应,为工厂化光设施育苗冬季补光提供理论指导。
    方法 以茶树品种‘谷雨春’为材料,设置远红光处理(FR,R/FR=4.1)和白光处理(CK,R/FR=10.4),研究低温条件(10 ℃)下不同R/FR比值光环境对茶树气体交换参数、荧光参数及光敏色素互作因子(phytochrome interacting factors, PIFs)表达量的影响。
    结果 低温条件下添加远红光,降低R/FR比值后,导致茶树叶片蒸腾速率(Tr)、净光合速率(Pn)与电子传递速率(ETR)下降,非调节性能量耗散的量子产量Y(NO)所占的比例升高,实际量子产量(YⅡ)和调节性能量耗散的量子产量Y(NPQ)所占的比例下降,叶绿素合成的重要调节因子CsPIF3a基因下调表达。
    结论 低温条件下低比值的R/FR光环境削弱了茶树叶片的光合电子传递能力,抑制叶绿素合成相关基因的表达,降低光合能力,叶片受到光损伤。

     

    Abstract:
    Objective The physiological and molecular responses of tea plants under different red/far-red light ratio ( R/FR ) light environments under low-temperature conditions were explored to provide theoretical guidance for winter light supplementation in factory light facilities.
    Method ‘Guyuchun’ was used as the material, and far red light treatment (FR, R/FR = 4.1) and white light treatment (CK, R/FR = 10.4) were set up to study the effects of different R/FR ratio light environments on gas exchange parameters, fluorescence parameters, and the expression of phytochrome interaction factors (PIFs) of tea plants under low-temperature conditions (10 °C).
    Result The transpiration rate (Tr), net photosynthetic rate (Pn), and electron transport rate (ETR) of tea leaves decreased. the proportion of non-regulated energy dissipation quantum yield Y (NO) increased, the proportion of actual quantum yield (YII) and regulated energy dissipation quantum yield Y (NPQ) decreased, and the important regulator of chlorophyll synthesis CsPIF3a gene was down-regulated.
    Conclusion The low ratio of R: FR light environment under low temperature conditions weakens the photosynthetic electron transport capacity of tea leaves, inhibited the expression of chlorophyll synthesis related genes, reduces the photosynthetic capacity, and leaves are subjected to light damage.

     

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