• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

一株以肝脾白色坏死点为特征的鹅源番鸭呼肠孤病毒的分离鉴定

Isolation and identification of a goose-origin muscovy duck reovirus strain causing gosling white point necrotic in liver and spleen

    摘要
  • 摘要:
    目的 通过对雏鹅以肝脾白色坏死点为特征的病料进行病原分离与鉴定，为该病的防控提供科学依据。
    方法 利用PCR检测排查病原，挑选阳性病料接种番鸭胚和番鸭成纤维细胞（Muscovy duck embryo fibroblast cells, MDEF）进行病毒分离，对分离毒再进行RT-PCR鉴定、病毒关键基因序列分析、动物回归试验验证。
    结果 成功分离出一株鹅源番鸭呼肠孤病毒（Goose-origin Muscovy reovirus, Go-MDRV），命名为JS2022株。分离毒接种番鸭胚至第6代时，番鸭胚死亡时间稳定在3～5 d，胚体出血、肝脏有大小不一的出血点和坏死灶、发育受阻。将分离毒尿囊液接种MDEF，出现细胞圆缩、崩解等病变。将JS2022株σB和σC基因的核苷酸序列与鹅源番鸭呼肠孤病毒、番鸭呼肠弧病毒（Muscovy duck reovirus, MDRV）、新型鸭呼肠孤病毒（Novel duck reovirus, NDRV）和禽呼肠孤病毒（Avian reovirus, ARV）等毒株序列进行同源性比对，结果JS2022株与鹅源番鸭呼肠孤病毒毒株的同源性最高，核苷酸同源性范围分别为99.1%～99.5%和99.3%～99.9%；动物回归试验成功复制出与临床发病鹅相同的病症。
    结论 本试验成功从病死鹅的肝脏和脾脏中分离鉴定1株鹅源番鸭呼肠孤病毒 JS2022株，为鹅源番鸭呼肠孤病毒病的防治提供参考价值。

     

    Abstract: :
    Objective To isolate and identify the causative agent of the gosling disease characterized by white pin-head necrotic foci in the liver and spleen, providing the basis for the disease’s prevention and control.
    Methods PCR was used to identify the pathogen, and positive samples were selected for virus isolation by inoculating them in duck embryos and muscovy duck embryo fibroblasts (MDEF) cells, and the isolated viruses were identified by RT-PCR, viral genes sequencing, and birds challenge tests.
    Results A goose-origin muscovy reovirus (Go-MDRV) strain was successfully isolated and named JS2022. After 6 th viarl passage, the death time of muscovy duck embryo was stable at 3-5 days post inoculation. The dead embryos were stunted, haemorrhagic spots and necrotic foci were observed in the liver.After inoculation with JS2022, the MDEF cells developed cytopathic effect characterized by cell shrinkage and disintegration.The nucleotide homology of σB and σC genes of JS2022 were compared withthose of Go-MDRV, muscovy duck reovirus (MDRV), novel duck reovirus (NDRV), and Avian reovirus (ARV), respectively. The results showed that the σB and σC genes of JS2022 had the highest homology with those of Go-MDRV, with nucleotide homology ranging from 99.1% to 99.5% and 99.3% to 99.9%, respectively. The birds of artificial challenge showed the similar symptoms with clinical cases.
    Conclusion In this study, a strain of Go-MDRV was successfully isolated and identified, providing valuable reference for this disease’s prevention and control.

     

    • HTML全文
    • 参考文献(23)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回