• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

艾BES1基因家族的鉴定及激素响应分析

Genome-wide identification and hormone response profiling of AaBES1 gene family in Artemisia argyi

  • 摘要: :【目的】从艾全基因组数据中鉴定出AaBES1基因家族成员。对其理化性质、启动子元件、系统进化关系和表达模式进行分析,研究AaBES1基因家族的表达特性。【方法】使用在线分析软件对艾BES1家族进行生物信息学分析;利用转录组数据,明确各成员在艾不同组织的表达水平以及响应盐碱胁迫下的表达情况;并采用实时荧光定量PCR(qRT-PCR)技术对AaBES1成员进行艾不同组织和应对激素处理的表达模式进行分析。【结果】从艾基因组中共鉴定获得14个BES1基因,AaBES1s的亚细胞定位均定位在细胞核;进化树分为三类;AaBES1基因家族启动子含有多种逆境和激素响应元件;BES1家族成员响应盐碱胁迫,既存在正调控,又有负调控成员,表现出不同成员响应逆境胁迫的多样性;该家族成员在艾不同组织中的表达模式具有组织特异性;在应对吲哚乙酸、脱落酸、茉莉酸甲酯和水杨酸处理时,基因AaBES1-2、AaBES1-9和AaBES1-11表现对激素处理较为敏感,在处理早期参与调控,处理48h后AaBES1-2在吲哚乙酸、脱落酸、茉莉酸甲酯处理后基因表达量显著上调,而AaBES1-9在脱落酸、茉莉酸甲酯和水杨酸处理后明显下调。【结论】通过对艾BES1基因家族进行鉴定和激素应答分析,表明艾BES1基因家族在植物激素应答过程中发挥重要作用,并为进一步开展艾BES1基因功能的

     

    Abstract: Abstract: 【Objective】 The members of the AaBES1 gene family were identified from the Artemisia argyi Genome-wide data. Their physicochemical properties, promoter elements, phylogenetic relationships and expressions pattern were analyzed to study the expression characteristics of the AaBES1 geng family. 【Methods】 Bioinformatics analysis was performed on the AaBES1 family using online analysis software. The transcriptome data were used to determine the expression level of each member in response to saline-alkali stress. Real-time quantitative PCR (qRT-PCR) was used to detect the expression patterns of AaBES1 members in different tissues and hormone treatments of A. argyi. 【Results】 A total of fourteen BES1 members were identified in this study, and subcellular localization results showed that the family was localized to the nucleus; Evolutionary trees are divided into three group; The promoter contains a variety of adversity and hormone-responsive elements; BES1 family members had both positive and negative regulatory members in response to salinity-alkali stress, showing the diversity of different members in response to adversity stress. The

     

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