Abstract:
Objective Sequence and annotation of Bacillus mojavensis JK-18 genome isolated from the rhizosphere soil of walnut trees were carried out, and gene clusters related to the synthesis of antimicrobial secondary metabolites were identified.
Method This study used PacBio technology to perform whole-genome sequencing of Bacillus mojavensis. Key information on the genome characteristics, gene function annotation, classification and secondary metabolites of the strain were obtained.
Results The results showed the genome of JK-18 is a circular closed DNA with a size of 4166746 bp, containing a total of 4138 protein-coding genes, and 4 kinds of CRISPR, The genome has 5844, 2299, 3257, 183 and 7 functional genes annotated in GO, KEGG, EGGNOG, CAZY and CARD databases, respectively. The secondary metabolites genes of JK-18 were predicted by antiSMASH. The gene clusters related to the synthesis of antimicrobial secondary metabolites, including polyketide compound such as lichenysin, macrobrevin and aurantinin, peptidolipid such as surfactin, bacillomycin, plipastatin and fengycin, and macrolides such as dumulmycin and dactylocycline A were predicted.
Conclusion The whole genome sequence of this strain reported here will be a valuable reference for studying the biocontrol mechanisms of this strain on walnut trees blight.