Abstract:
Objective In order to study the proteins related to the response of Duyun Maojian native tea plant infected by Empoasca vitis Gothe.
Method The leaves of Duyun Maojian native tea plant were infected by E. vitis Göthe for 0 h, 12 h, 24 h, 36 h and 48 h, were used as research materials. Tandem mass tag (TMT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was used to qualitatively and quantitatively analyze the proteins of tea leaves infected with E. vitis Göthe.
Results We identified a total of 2,893 proteins. There were 0, 1, 848, and 849 differential expression proteins (DEPs) between 0 h and 12 h, 0 h and 24 h, 0 h and 36 h, 0 h and 48 h, respectively. Furthermore, 2,622 proteins were annotated, of which 2,360 proteins were annotated to the GO database and 1,232 proteins were annotated to KEGG database. GO and KEGG analyses showed that tea plants may be in resistance to E. vitis Göthe infestation through CMK, FPPS, GPPS, GGPPS, peroxidase, pectinesterase, and allene-oxide cyclase, sesquiterpenoid, triterpeoid, monoterpenoid, diterpenoid, sesquiterpenoid and triterpenoid biosynthesis, terpenoid backbone biosynthesis, and monoterpenoid biosynthesis.
Conclusion Four DEPs (CMK, FPPS, GPPS, and GGPPS) and terpenoids may play important roles in the response and defense against E. vitis Göthe. The research results may provide theoretical basis for revealing the molecular mechanism of tea plant response to the harm of E. vitis Göthe.
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