基于MAS技术提高水稻恢复系明恢1831的碱消值

邹文广; 候新坡; 马彬林; 卓伟; 杨旺兴; 杨腾帮; 许旭明

基于MAS技术提高水稻恢复系明恢1831的碱消值

Improving Alkali Spreading Value of Minghui 1831 Rice by Means of MAS-assisted Technology

摘要

摘要:
目的通过分子标记辅助选择提高抗稻瘟病香型恢复系明恢1831的稻米碱消值，以提高其在食味品质育种中的利用价值。
方法通过常规回交育种、分子标记辅助选择和稻米碱消值检测相结合的手段，将供体亲本明香510的高碱消值基因导入明恢1831中。用水稻48对SSR引物分析改良系与明恢1831的遗传相似性，并对改良系、明恢1831及其与明太A、甜香2A等4个不育系配制组合进行主要农艺性状和部分品质性状比较。
结果在BC₃F₂群体中用ZiS_1_ALK分子标记检测到8个纯合高碱消值优良株系，其中MG-2、MG-3、MG-6等3个株系与轮回亲本明恢1831遗传背景相似，部分农艺性状和米粒长宽比优于轮回亲本。杂交组合分析结果表明，提高明恢1831的碱消值并未影响其配合力。
结论筛选获得3份遗传了明恢1831的优良特点和配合力的高碱消值株系，对明恢1831的碱消值改良是非常成功的。

Abstract:
Objective Molecular marker-assisted selection (MAS) was applied in breeding to improve the alkali spreading value (ASV) of Minghui 1831.
Method The high-ASV gene of Mingxiang 510 was introduced to the blast-resistant, aromatic Minghui 1831 using backcross breeding, MAS, and ASV detection. Genetic similarity between Minghui 1831 and the improved lines was analyzed based on 48 pairs of SSR primers. Main agronomic and some quality traits of them, as well as the combinations between Minghui 1831 and 4 sterile lines including Mingtai A and Tianxiang 2A, were compared.
Results The ZiS_1_ALK molecular markers in the BC₃F₂ population were used to identify 8 homozygous high-ASV lines. Of which, MG-2, MG-3, and MG-6 were similar in genetic background to the recurrent parent, Minghui 1831, while superior on some agronomic traits and grain length/width ratio. Meanwhile, the combining ability remained intact.
Conclusion Three newly bred high-ASV rice lines were successfully obtained that inherited the desirable characteristics and combining ability of their parent, Minghui 1831.

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