Objective EST-SSR markers were developed based on lemon transcriptome data and genetic diversity was analyzed.

Method The SSR loci of lemon transcriptome were searched by MISA software,and the primers were designed and screened by Primer3.0,and the cluster diagram of 49 lemons was drawn.

Result A total of 40193 SSR loci were identified,with a distribution frequency of 35.67 % and an average distribution distance of 1.39 kb.The SSR sequences of lemon transcriptome were mainly mono-,tri- and dinucleotide repeat types,comprising 80.91 %,9.20 % and 8.67 %,respectively.The dominant motifs were A / T,AG / CT and AAG / CTT,respectively.A total of 4561 pairs of primers were designed,of which 24 pairs were selected for validity verification,and 13 pairs of polymorphic primers were screened.A total of 47 alleles were amplified,and the mean effective allele was 3.615. The mean values of Ho,He,and PIC were 0.637,0.516 and 0.528,respectively.When the genetic distance was 0.40,the tested materials could be divided into five categories,and the clustering results were generally consistent with the traditional morphological classification results.

Conclusion In this study, 13 pairs of EST-SSR primers were developed to distinguish the tested materials, which was consistent with the traditional taxonomy, and revealed the rich genetic diversity among the tested materials, which provided a reference for the subsequent development and utilization of lemon germplasm resources.