Abstract:
Objective The deletion of either OsPLGG1a or OsPLGG1b in the GOC rice engineering optimizes the photorespiration pathway, thereby enhancing rice photosynthesis efficiency and optimizing photorespiration metabolic engineering.
Method The knockout vectors osplgg1a-Cas9 or osplgg1b-Cas9 were constructed and transformed into GOC rice, respectively, to obtain GOC transgenic homozygous lines with deletion of OsPLGG1a or OsPLGG1b, and the photosynthesis rate was measured.
Result Transgenic homozygous lines with the GOC rice of OsPLGG1a or OsPLGG1b knocked out were obtained. The phenotypes of osplgg1a-GOC plants were similar to osplgg1a, both of which showed stunted growth. Compared with GOC rice, the net photosynthetic rate of osplgg1b-GOC plants increased, indicating that OsPLGG1b mutation was conducive to the retention of chloroplast glycolate, increased the metabolic flux through the GOC bypass, reduced the photorespiration metabolism of plants, and further increased the CO2 concentration in chloroplasts.
Conclusion Compared with GOC rice, the net photosynthetic rate and stomatal conductance of osplgg1b-GOC plants were increased, suggesting that the elimination of OsPLGG1b could be used to optimize the photorespiratory metabolic pathway.
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