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姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响

李林明 张子平 王若璇 黄一帆

李林明,张子平,王若璇,等. 姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响 [J]. 福建农业学报,2023,38(9):1030−1036 doi: 10.19303/j.issn.1008-0384.2023.09.004
引用本文: 李林明,张子平,王若璇,等. 姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响 [J]. 福建农业学报,2023,38(9):1030−1036 doi: 10.19303/j.issn.1008-0384.2023.09.004
LI L M, ZHANG Z P, WANG R X, et al. Effect of Curcumin on HSP Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress [J]. Fujian Journal of Agricultural Sciences,2023,38(9):1030−1036 doi: 10.19303/j.issn.1008-0384.2023.09.004
Citation: LI L M, ZHANG Z P, WANG R X, et al. Effect of Curcumin on HSP Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress [J]. Fujian Journal of Agricultural Sciences,2023,38(9):1030−1036 doi: 10.19303/j.issn.1008-0384.2023.09.004

姜黄素对过氧化氢诱导的罗非鱼肝细胞热休克蛋白基因表达的影响

doi: 10.19303/j.issn.1008-0384.2023.09.004
基金项目: 福建省高校领军人才项目(660160010);福建省自然科学基金项目(2023J01488)
详细信息
    作者简介:

    李林明(1985 —),男,博士,实验师,主要从事水产动物药理学相关研究,E-mail:444853510@qq.com

    通讯作者:

    张子平(1964 —),男,博士,教授,主要从事水产生物技术相关研究,E-mail: zhangziping@fafu.edu.cn

  • 中图分类号: S917.4

Effect of Curcumin on HSP Expression in Tilapia Hepatocytes under Hydrogen Peroxide Stress

  • 摘要:   目的  探究热休克蛋白基因在氧化应激和姜黄素作用下的表达差异,了解姜黄素药效的发挥与热休克蛋白基因表达变化的关系,进一步探讨热休克蛋白对氧化应激条件下鱼类肝细胞中的功能,为鱼类氧化应激的毒理学机制及姜黄素的药理学机制提供理论资料。  方法  对24 h姜黄素预处理(保护组)与无姜黄素预处理(对照组)的罗非鱼肝细胞分别进行0、1、2.5 h 的H2O2应激,并对肝细胞5种高分子热休克蛋白基因(HSP70、HSP90α、HSP90β、HSP60、HSP75)和5种小分子热休克蛋白基因(HSP30、HSPB1、 HSPB7、HSPB8、HSPB11)的表达水平进行qPCR检测。  结果  与不经H2O2处理(0 h)相比,H2O2作用 1 h能显著提升HSPB1表达量,降低HSP30表达量;H2O2作用2.5 h 能显著提升HSP90aHSPB1的表达量。姜黄素预处理24 h能显著提升HSP70HSP90αHSP30HSPB1的表达量;姜黄素预处理24 h后再进行H2O2应激1 h时,HSP70HSP90αHSP30的表达量显著高于H2O2单独应激(对照组);姜黄素预处理24 h后再进行H2O2应激2.5 h时,HSP90αHSP30HSPB1的表达量显著高于H2O2单独应激。其余热休克蛋白在试验过程中无显著差异。  结论  H2O2单独应激可引起罗非鱼肝细胞HSP90a、HSP30、HSPB1基因的表达量变化,而姜黄素预处理能提高多种热休克蛋白基因的表达,提高了罗非鱼肝细胞的抗氧化能力,最终抵御H2O2引起的氧化应激并维持细胞活力。
  • 图  1  姜黄素预处理对H2O2胁迫下罗非鱼肝细胞高分子HSP基因表达的影响

    *表示同一处理时间保护组与对照组间差异显著(P < 0.05),不同字母小写字母表示同一处理组中不同时间点之间相比差异显著(P < 0.05)。图2同。

    Figure  1.  Effect of curcumin pretreatment on HSP expressions of tilapia hepatocytes under H2O2 stress

    * indicates significant difference from control at relevant time point at P<0.05; data with different lowercase letters indicate significant differences at different time points in same group at P<0.05. Same for Fig. 2.

    图  2  姜黄素与H2O2处理对罗非鱼肝细胞sHSP基因表达的影响

    Figure  2.  Effect of curcumin pretreatment on sHSP expressions of tilapia hepatocytes under H2O2 stress

    表  1  qPCR检测所用的各基因引物

    Table  1.   Primers used for qPCR

    基因
    Gene
    长度
    Length/bp
    引物序列(5′–3′)
    Sequence (5′–3′)
    HSP70107HSP70F: CATCGCCTACGGTCTGGACAA
    HSP70R: TGCCGTCTTCAATGGTCAGGAT
    HSP90α128HSP90αF: ATTGCTCAGCTGATGTCCCT
    HSP90αR: GTGGGATCCGTCAAGCTTTC
    HSP90β117HSP90βF: AGAACCTCAAGCTGGGTGTG
    HSP90βR: TCACGTACTCCGAGAGGGAG
    HSP60115HSP60F: GCGGCTAGCAGTAAGCCTTT
    HSP60R: CGTAGGCTCGTGTGAGGTG
    HSP75103HSP75F: GTTGGGCCCACCATTCATCC
    HSP75R: TTCTCAACCATCCACGCCAT
    HSP30111HSP30F: GACTCTGGACCTCCTCCACT
    HSP30R: TGAAACACTCTTCAGGTGTCTGT
    HSPB1106HSPB1F: CTTTGACCAGACCTTCGGCA
    HSPB1R: TAGTTCTGGGGCCAGGATGG
    HSPB7119HSPB7F: ACTTTCACCCACAAGTGCCA
    HSPB7R: AGCTCATGCTTGGCTGGATT
    HSPB8104HSPB8F: TGGAAAGTCTGCGTGAACGTC
    HSPB8R: TCCTGTTTCTCTTCGTGCTTTC
    HSPB11100HSPB11F: CAGCTTGTTTGGCGATGACC
    HSPB11R: CCGGTTGAAGAAGTCTCGCT
    β-Actin129β-Actin-F: TGCGGGATATCATTTGCCTGA
    β-Actin-R: GAATCCGGCCTTGCACATAC
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-02-13
  • 修回日期:  2023-06-21
  • 网络出版日期:  2023-10-25
  • 刊出日期:  2023-09-28

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