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福建永泰茶树种质资源遗传多样性分析及DNA指纹图谱构建

王攀 于文涛 吕水源 朱艳宇 兰振良 李青颖 李国来 贵文静 叶乃兴

王攀,于文涛,吕水源,等. 福建永泰茶树种质资源遗传多样性分析及DNA指纹图谱构建 [J]. 福建农业学报,2024,39(1):66−74 doi: 10.19303/j.issn.1008-0384.2024.01.009
引用本文: 王攀,于文涛,吕水源,等. 福建永泰茶树种质资源遗传多样性分析及DNA指纹图谱构建 [J]. 福建农业学报,2024,39(1):66−74 doi: 10.19303/j.issn.1008-0384.2024.01.009
WANG P, YU W T, LV S Y, et al. Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian [J]. Fujian Journal of Agricultural Sciences,2024,39(1):66−74 doi: 10.19303/j.issn.1008-0384.2024.01.009
Citation: WANG P, YU W T, LV S Y, et al. Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian [J]. Fujian Journal of Agricultural Sciences,2024,39(1):66−74 doi: 10.19303/j.issn.1008-0384.2024.01.009

福建永泰茶树种质资源遗传多样性分析及DNA指纹图谱构建

doi: 10.19303/j.issn.1008-0384.2024.01.009
基金项目: 福建省农业科技引导性项目(2021N0024);海关总署科研项目(2020HK187);福建农林大学横向课题(KH220008A);福建张天福茶叶发展基金会科技创新基金(FJZTF01)
详细信息
    作者简介:

    王攀(1996 —),男,硕士研究生,主要从事茶树栽培育种研究,E-mail:3210330055@fafu.edu.cn

    通讯作者:

    于文涛(1984 —),男,博士,正高级农艺师,主要从事植物种质资源研究,E-mail: wtyu@foxmail.com

    叶乃兴(1963 —),男,硕士,教授,主要从事茶树栽培育种与茶叶品质化学研究,E-mail: ynxtea@126.com

  • 中图分类号: S571.1

Genetic Diversity and DNA Fingerprints of Tea Germplasms in Yongtai, Fujian

  • 摘要:   目的  分析永泰县茶树种质资源的遗传多样性及群体结构,探明不同茶树资源群体的亲缘关系,为当地茶树种质资源的收集和优异种质利用提供依据。  方法  利用 EST-SNP 分子标记技术对77份永泰及其周边地区茶树种质资源进行亲缘关系和遗传多样性分析。  结果  筛选出48个适用于鉴定永泰茶树种质的SNP位点。这些 SNP 位点的多态性信息指数平均值为0.407,观测杂合度平均值为0.303,期望杂合度平均值为0.271,固定指数平均值为−0.092,次等位基因频率平均值为0.268。通过主坐标分析、聚类分析和 Structure 群体结构分析,发现永泰茶树种质的同一群体内部的交流多于不同群体之间的交流,且梧桐的茶树种质与洑口、丹云等地的种质差异较大,两者间的遗传距离较远,但又与尤溪和大田的茶树亲缘关系较近。此外,应用筛选出的48个高质量 SNP 位点构建了54份永泰茶树种质的 DNA 指纹图谱,可用于相关茶树种质的精确鉴定。  结论  永泰县茶树种质资源多样性丰富;永泰县茶树种质资源按照地理位置可划分为梧桐镇产区和其他地区两个类群;梧桐茶树资源与大田和尤溪的茶树资源亲缘关系较为密切,而洑口、丹云等地的茶树资源与永泰县北部产茶地的茶树资源亲缘关系更近。
  • 图  1  48个多态性SNP标记的次要等位基因频率

    Figure  1.  Frequency on minor alleles of 48 polymorphic SNP markers

    图  2  永泰茶树种质资源的PCoA 分析

    A:基于前三个主成分的 77份茶树种质资源 PCoA 图;B:基于前三个主成分的54份永泰县内茶树种质资源 PCoA图。

    Figure  2.  PCoA of tea germplasms from Yongtai

    A: PCoA plot of 77 tea germplasms based on top three principal components; B: PCoA plot of 54 germplasms from Yongtai based on top three principal components.

    图  3  永泰茶树种质资源的系统发育树与群体结构

    K的比值=K的似然性二阶变化率的平均值/K 的似然度标准偏差;A:N-J树;B:层级树;C:K的比值;D:群体结构。

    Figure  3.  Phylogenetic tree and population structure of tea germplasms from Yongtai County

    DeltaK=mean(|L"(K)|)/sd[L(K)]; A: Neighbor-joining tree; B: dendrogram on relationship among germplasms; C: Delta K; D: model-based germplasm structures.

    图  4  永泰茶树种质基于EST-SNP的DNA指纹图谱

    Figure  4.  DNA fingerprints of Yongtai tea germplasms based on EST-SNP

    表  1  用于SNP基因分型的供试茶树种质

    Table  1.   Germplasms used for SNP genotyping

    来源
    Source
    样品名称
    Sample name
    份数
    Numbers
    永泰北部相邻产茶区
    Adjacent tea producing areas in the northern part of Yongtai
    福州晋安 JA:鼓山1号~鼓山6号(01-06);
    福州长乐 CL:长乐1号~长乐5号(07-11)
    11
    永泰丹云 DY丹云1号~丹云11号(12-22)11
    永泰洑口 FK洑口1号~洑口24号(23-46)24
    永泰梧桐 WT梧桐1号~梧桐19号(47-65)19
    永泰南部相邻产茶区
    Adjacent tea producing areas in the southern part of Yongtai
    大田 DT:大田1号~大田3号(66-68);尤溪 YX:
    尤溪1号~尤溪3号(69-71);德化 DH:德化1号~德化6号(72-77)
    12
    下载: 导出CSV

    表  2  48个多态性SNP位点的等位基因信息

    Table  2.   Information on alleles of 48 polymorphic SNP markers

    位点 Locus信息指数 I观测杂合度 Ho期望杂合度 He固定指数 F位点 Locus信息指数 I观测杂合度 Ho期望杂合度 He固定指数 F
    cs1 0.453 0.326 0.299 −0.100 cs134 0.657 0.759 0.465 −0.620
    cs115 0.622 0.527 0.432 −0.211 cs23 0.227 0.136 0.139 −0.036
    cs15 0.381 0.281 0.244 −0.131 cs33 0.354 0.246 0.238 −0.027
    cs167 0.441 0.410 0.301 −0.341 cs55 0.419 0.425 0.284 −0.430
    cs3 0.327 0.146 0.194 0.106 cs76 0.291 0.227 0.183 −0.227
    cs84 0.339 0.158 0.219 0.160 cs112 0.580 0.341 0.394 0.171
    cs10 0.602 0.677 0.415 −0.570 cs139 0.531 0.378 0.353 −0.088
    cs170 0.560 0.490 0.393 −0.230 cs191 0.379 0.106 0.261 0.607
    cs202 0.566 0.406 0.382 −0.101 cs36 0.555 0.437 0.389 −0.130
    cs51 0.435 0.415 0.286 −0.352 cs113 0.355 0.193 0.228 0.146
    cs85 0.252 0.167 0.151 −0.092 cs122 0.471 0.529 0.329 −0.573
    cs104 0.349 0.043 0.237 0.711 cs141 0.405 0.417 0.274 −0.432
    cs131 0.576 0.298 0.393 0.212 cs198 0.436 0.269 0.276 −0.018
    cs207 0.379 0.250 0.250 −0.014 cs213 0.310 0.231 0.201 −0.104
    cs219 0.220 0.169 0.141 0.054 cs26 0.210 0.153 0.127 −0.183
    cs31 0.280 0.240 0.181 −0.256 cs39 0.583 0.289 0.399 0.266
    cs44 0.544 0.452 0.361 −0.182 cs48 0.221 0.187 0.144 −0.211
    cs88 0.525 0.343 0.361 0.068 cs66 0.635 0.566 0.445 −0.269
    cs132 0.197 0.140 0.118 −0.168 cs114 0.213 0.096 0.130 0.198
    cs180 0.427 0.308 0.275 −0.143 cs146 0.176 0.120 0.103 −0.140
    cs208 0.340 0.288 0.218 −0.242 cs20 0.170 0.101 0.102 −0.021
    cs32 0.499 0.263 0.336 0.210 cs215 0.540 0.479 0.376 −0.254
    cs54 0.441 0.285 0.289 −0.047 cs49 0.419 0.357 0.272 −0.258
    cs9 0.468 0.294 0.313 0.084 cs81 0.170 0.133 0.107 −0.222
    下载: 导出CSV

    表  3  永泰与周边地区茶树种质资源群体间遗传分化指数和遗传距离

    Table  3.   Fixation indices and genetic distance of different tea populations in Yongtai

    区域
    Areas
    晋安
    JA
    长乐
    CL
    丹云
    DY
    洑口
    FK
    梧桐
    WT
    大田
    DT
    尤溪
    YX
    德化
    DH
    晋安 JA0.0710.0400.0360.1650.1330.2120.078
    长乐 CL0.0790.0480.0430.1610.1030.2180.109
    丹云 DY0.0370.0500.0280.1720.1150.2190.088
    洑口 FK0.0350.0500.0230.1700.1090.2080.078
    梧桐 WT0.2030.1910.2130.2090.0880.1600.141
    大田 DT0.1520.1020.1220.1240.0700.2120.113
    尤溪 YX0.2200.2210.2270.2250.1210.1600.192
    德化 DH0.0860.1250.0980.0860.1550.1140.182
    上三角数据为种群间遗传分化指数(Fst);下三角数据为种群间遗传距离(GD)。
    Above diagonal: Fst; below diagonal : Neis genetic distance (GD).
    下载: 导出CSV

    表  4  77份茶树种质资源的AMOVA分析

    Table  4.   AMOVAs of 77 tea germplasms

    差异来源
    Source of variation
    自由度
    Mean of Square
    平方和
    Sum of Square
    平均平方
    Mean of Square
    估算方差
    Estimated variance
    百分比 /%
    种群之间 Among Pops7526.67475.2396.91433
    种群内部 Within Pops69981.79414.22914.22967
    合计 Total761508.46889.46821.143100
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-09-08
  • 修回日期:  2023-11-14
  • 网络出版日期:  2024-01-06
  • 刊出日期:  2024-01-28

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