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多花黄精种子生理后熟过程的生理变化及基因表达模式分析

苏海兰 牛雨晴 成建华 朱雁鸣 陈宏 郑梅霞 黄贤贵 朱育菁

苏海兰,牛雨晴,成建华,等. 多花黄精种子生理后熟过程的生理变化及基因表达模式分析 [J]. 福建农业学报,2024,39(6):662−670 doi: 10.19303/j.issn.1008-0384.2024.06.005
引用本文: 苏海兰,牛雨晴,成建华,等. 多花黄精种子生理后熟过程的生理变化及基因表达模式分析 [J]. 福建农业学报,2024,39(6):662−670 doi: 10.19303/j.issn.1008-0384.2024.06.005
SU H L, NIU Y Q, CHENG J H, et al. Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment [J]. Fujian Journal of Agricultural Sciences,2024,39(6):662−670 doi: 10.19303/j.issn.1008-0384.2024.06.005
Citation: SU H L, NIU Y Q, CHENG J H, et al. Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment [J]. Fujian Journal of Agricultural Sciences,2024,39(6):662−670 doi: 10.19303/j.issn.1008-0384.2024.06.005

多花黄精种子生理后熟过程的生理变化及基因表达模式分析

doi: 10.19303/j.issn.1008-0384.2024.06.005
基金项目: 福建省科技重大专项(2022NZ029017);福建省科技计划公益类专项(2023R1032003);福建省农业科学院英才项目(YC2021002);福建省农业科学院科技创新团队建设项目(2022KF01-1);南平市科技计划项目(N2022T021)
详细信息
    作者简介:

    苏海兰(1980 — ),女,硕士,高级农艺师,主要从事药用植物资源利用与栽培研究,E-mail:suhailan2019@163.com

    通讯作者:

    黄贤贵(1965 —),男,研究员,主要从事园艺作物及药用植物研究,E-mail:hxg323@163.com

    朱育菁(1972 — ),女,博士,研究员,主要从事农业生物资源保护与利用研究,E-mail:zyjingfz@163.com

  • 中图分类号: R28

Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment

  • 摘要:   目的  探明多花黄精种子生理后熟过程中生理生化变化规律及差异基因表达情况。  方法  以多花黄精种子生理后熟过程6个阶段的种子为材料,采用化学测定方法,分析每个阶段的超氧化物歧化酶、α淀粉酶、β淀粉酶、可溶性蛋白、粗脂肪、可溶性糖含量。采用高通量转录组测序技术,对6个阶段的差异表达基因进行分析,并用qRT-PCR法对关键差异基因的表达量进行验证。  结果  (1)多花黄精种子生理后熟过程中,可溶性糖含量在前4个时期逐渐增高后,在后两个时期稍有下降;粗脂肪、蛋白质整体呈下降趋势;α-淀粉酶活性及β-淀粉酶活性呈现增强趋势。(2)多花黄精种子生理后熟过程中,蔗糖和淀粉代谢途径、激素信号转导通路等显著富集差异表达基因。  结论  多花黄精种子生理后熟过程中,淀粉等营养物质的代谢、转化与内源激素的协调作用,促进多花黄精种子的生理后熟。
  • 图  1  多花黄精种子生理后熟过程的表型分析

    T0,后熟休眠期;T1,初始萌发期;T2,完全萌发期;T3,小球茎形成期;T4,胚芽形成期;T5,初生根茎形成期。

    Figure  1.  Superficial analysis on physiological post-ripening process of P. cyrtonema Hua seeds

    T0: post-mature dormancy; T1: initial germination stage; T2: complete germination stage; T3: corm formation stage; T4: germ formation stage; T5: primary rhizome formation stage.

    图  2  多花黄精种子生理后熟过程中营养物质与酶的动态变化

    Figure  2.  Dynamic changes on nutrients and enzymes in P. cyrtonema Hua seeds during post-ripening treatment

    图  3  多花黄精种子生理后熟过程的转录组测序分析

    a:差异表达基因数量; b:主成分分析;c~e:T0vsT1, T0vsT2, T0vsT3, T0vsT4和T0vsT5的共有、上调和下调差异基因韦恩图。

    Figure  3.  Transcriptome sequencing on physiological post-ripening process of P. cyrtonema Hua seeds

    a: number of differentially expressed genes; b: principal component analysis; c–e: shared, upregulated, and downregulated differential genes of 5 comparison groups (T0 vs. T1, T0 vs. T2, T0 vs. T3, T0 vs. T4, and T0 vs. T5), respectively.

    图  4  差异表达基因的KEGG富集分析

    Figure  4.  Statistics of KEGG enrichment analysis on DEGs

    图  5  植物激素信号转导通路基因表达模式

    红色方框表示基因的上调,绿色方框表示基因的下调,黄色方框表示同时存在上调和下调基因。

    Figure  5.  Gene expressions of plant hormone signaling pathways

    Red, green, yellow boxes indicate up-regulated, down-regulated and both up-regulated and down-regulated genes.

    图  6  差异基因的表达分析

    Figure  6.  Expression analysis on differential genes

    表  1  荧光定量PCR引物序列

    Table  1.   Sequences of qPCR primers

    基因名称
    Gene name
    正向引物
    Forward primer (5'-3')
    反向引物
    Reverse prime (5'-3')
    TGA10 TGGTCGCCAATAATGCCTCC GAAGGCTTGCGCAGAATAGG
    IAA18 TTGCTGCTGGGAATCAGAGG ATGCGGTCTCCTTCATCGTC
    IAA30 TGCGGTACATCTTGAGGTCG GAGGAGTCCAGCACCAATCC
    TIFY 10 TTTCGGATTCGGGTAAGCCC CTGAATTCGGGTTCGGCTCT
    GAPDH GCCTGTGGTTATCACTGCCT CGGATCGTCTGCTCCACTAC
    下载: 导出CSV
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  • 收稿日期:  2024-01-11
  • 修回日期:  2024-03-18
  • 网络出版日期:  2024-06-26
  • 刊出日期:  2024-06-28

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