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不同紫云英品种根瘤内生细菌的类群和多样性分析

方宇 刘彩玲 林陈强 陈济琛 贾宪波

方宇,刘彩玲,林陈强,等. 不同紫云英品种根瘤内生细菌的类群和多样性分析 [J]. 福建农业学报,2024,39(6):730−737 doi: 10.19303/j.issn.1008-0384.2024.06.012
引用本文: 方宇,刘彩玲,林陈强,等. 不同紫云英品种根瘤内生细菌的类群和多样性分析 [J]. 福建农业学报,2024,39(6):730−737 doi: 10.19303/j.issn.1008-0384.2024.06.012
FANG Y, LIU C L, LIN C Q, et al. Endophytic Microbes in Root Nodules of Different Cultivars of Astragalus Sinicus L. [J]. Fujian Journal of Agricultural Sciences,2024,39(6):730−737 doi: 10.19303/j.issn.1008-0384.2024.06.012
Citation: FANG Y, LIU C L, LIN C Q, et al. Endophytic Microbes in Root Nodules of Different Cultivars of Astragalus Sinicus L. [J]. Fujian Journal of Agricultural Sciences,2024,39(6):730−737 doi: 10.19303/j.issn.1008-0384.2024.06.012

不同紫云英品种根瘤内生细菌的类群和多样性分析

doi: 10.19303/j.issn.1008-0384.2024.06.012
基金项目: 福建省科技计划公益类专项(2022R1025006);福建省自然科学基金项目(2023J01360、2021J01480);福建省农业科学院科技创新团队建设项目(CXTD2021002-3);福建省农业科学院农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021009);福建省农业科学院科技创新平台专项(CXPT2023007);现代农业产业技术体系建设专项(CARS-22);福建省对外合作项目(DWHZ2023-5)
详细信息
    作者简介:

    方宇(1979 —),女,博士,副研究员,主要从事农业微生物研究,E-mail:fangyu1106@163.com

    通讯作者:

    贾宪波 (1986 —),男,博士,副研究员,主要从事农业微生物研究,E-mail: xbj2011@163.com

  • 中图分类号: Q939.96

Endophytic Microbes in Root Nodules of Different Cultivars of Astragalus Sinicus L.

  • 摘要:   目的  阐释紫云英根瘤内生菌的群落结构,为研发根瘤菌菌剂及了解固氮机制提供依据。  方法  以福建省种植的不同紫云英品种的根瘤为材料,借助Illumina Miseq PE300测序平台,以16S rRNA基因为靶标,研究不同紫云英品种的根瘤内生细菌的多样性。  结果  不同品种会影响紫云英根瘤内生细菌的类群和多样性。浙紫5号(ZZ5)的优势属为假单胞菌属(Pseudomonas),其余品种根瘤内生细菌的优势属均为中慢生根瘤菌属(Mesorhizobium)。闽紫8号(MZ8)、信阳(XY)和浙紫5号的根瘤内生细菌的多样性指数(Shannon)较高,除了共生固氮的中慢生根瘤菌外,还包括丰富的非根瘤菌,如假单胞菌属、寡养单胞菌属(Stenotrophomonas)、杜擀氏菌属(Duganella)、泛菌属(Pantoea)、欧文氏菌属(Erwinia)等。闽紫8号、信阳和浙紫5号这3个品种根瘤中含有较高丰度的致病菌大黄欧文氏菌(Erwinia rhapontici)和菠萝泛菌PA13( Pantoea ananatis PA13)。  结论  紫云英品种对根瘤内生细菌的群落结构有一定影响,浙紫5号的根瘤内生细菌主要为具促生作用的非根瘤菌假单胞菌,而其余品种为具固氮能力的根瘤菌,在生产实际中可以采用紫云英混种的方式达到固氮促生的双重功效。
  • 图  1  不同紫云英品种根瘤内生细菌门水平的相对丰度

    MZ7:闽紫7号;MZ8:闽紫8号;GZ:光泽;XY:信阳;XZ:湘紫;YJZ:弋江籽;ZZ5:浙紫5号;下同。

    Figure  1.  Relative abundance of endophytic microbes at phylum level in root nodules of different cultivars of A. sinicus

    MZ7: Minzi No. 7; MZ8: Minzi No.8; GZ: Guangze; XY: Xinyang; XZ: Xiangzi; YJZ: Yijiangzi; ZZ5: Zhezi No. 5. Same for below.

    图  2  不同紫云英品种根瘤内生细菌目水平的相对丰度

    Figure  2.  Relative abundance of endophytic microbes at order level in root nodules of different cultivars of A. sinicus

    图  3  不同紫云英品种根瘤内生细菌属水平的相对丰度

    Figure  3.  Relative abundance of endophytic microbes at genus level in root nodules of different cultivars of A. sinicus

    图  4  不同紫云英品种根瘤内生细菌种水平的相对丰度

    Figure  4.  Relative abundance of endophytic microbes at species level in root nodules of different cultivars of A. sinicus

    图  5  基于OTU水平紫云英根瘤内生细菌群落组成主坐标分析

    Figure  5.  Principal coordinates analysis on endophytic microbes at OTU level in root nodules of different cultivars of A. sinicus

    表  1  不同紫云英品种根瘤内生细菌的Alpha多样性指数

    Table  1.   Alpha diversity index of endophytic microbes at genus level in root nodules of different cultivars of A. sinicus

    样本
    Sample
    Ace指数
    Ace index
    Chao1 指数
    Chao1 index
    Shannon指数
    Shannon index
    Simpson指数
    Simpson index
    覆盖度
    Coverage/%
    闽紫7号 MZ7 26.26 24.25 0.07 0.98 99.98
    闽紫8号 MZ8 104.42 90.11 1.58 0.30 99.93
    光泽 GZ 25.86 23.67 0.08 0.98 99.98
    信阳 XY 191.81 191.50 1.73 0.40 99.92
    湘紫 XZ 97.85 68.60 0.14 0.96 99.94
    弋江紫 YJZ 23.60 23.00 0.11 0.96 99.98
    浙紫5号 ZZ5 72.45 84.50 1.13 0.53 99.95
    下载: 导出CSV
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出版历程
  • 收稿日期:  2024-03-14
  • 修回日期:  2024-05-07
  • 网络出版日期:  2024-07-10
  • 刊出日期:  2024-06-28

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