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内蒙古赤峰地区辣椒病毒种类鉴定及其遗传进化特征

周艳芳 王秀芝 张晓梅 罗香文 刘泽红 崔聪聪 孟令强 孙军玲

周艳芳,王秀芝,张晓梅,等. 内蒙古赤峰地区辣椒病毒种类鉴定及其遗传进化特征 [J]. 福建农业学报,2024,39(7):819−825 doi: 10.19303/j.issn.1008-0384.2024.07.009
引用本文: 周艳芳,王秀芝,张晓梅,等. 内蒙古赤峰地区辣椒病毒种类鉴定及其遗传进化特征 [J]. 福建农业学报,2024,39(7):819−825 doi: 10.19303/j.issn.1008-0384.2024.07.009
ZHOU Y F, WANG X Z, ZHANG X M, et al. Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia [J]. Fujian Journal of Agricultural Sciences,2024,39(7):819−825 doi: 10.19303/j.issn.1008-0384.2024.07.009
Citation: ZHOU Y F, WANG X Z, ZHANG X M, et al. Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia [J]. Fujian Journal of Agricultural Sciences,2024,39(7):819−825 doi: 10.19303/j.issn.1008-0384.2024.07.009

内蒙古赤峰地区辣椒病毒种类鉴定及其遗传进化特征

doi: 10.19303/j.issn.1008-0384.2024.07.009
基金项目: 国家大宗蔬菜产业技术体系赤峰综合试验站(CARS-23-G21);湖南省自然科学基金(2021JJ30415);国家自然科学基金(32072383);湖南省科技创新项目(2022CX08、2023CX39)
详细信息
    作者简介:

    周艳芳(1986 — ),女,硕士,副研究员,主要从事植物病虫害研究,E-mail: yecaode@126.com

    通讯作者:

    王秀芝(1964 — ),女,研究员,主要从事辣椒新品种选育研究,E-mail: yzhdx@163.com

  • 中图分类号: S436.418.1+2

Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia

  • 摘要:   目的  系统检测内蒙古赤峰市辣椒主产区病毒发生种类,并分析病毒的遗传分化情况,以期为该地区辣椒病毒病精准防控提供理论依据。  方法  从内蒙古赤峰地区4个辣椒主产区采集疑似病毒侵染的辣椒样本47份,采用sRNA高通量测序技术结合生物信息学分析,以及常规RT-PCR检测,明确侵染辣椒的病毒种类及复合侵染情况;采用常规Sanger测序测定常规RT-PCR扩增片段序列,进行遗传进化分析。  结果  内蒙古赤峰地区4个辣椒主产区辣椒混合病样的sRNA高通量测序,共检测到6种病毒,常规RT-PCR共验证到其中4种病毒;4个地区病毒种类存在明显差异,松山区城子乡优势种类为蚕豆萎蔫病毒2(Broad bean wilt virus 2,BBWV2)和马铃薯Y病毒(Potato virus Y, PVY),喀喇沁旗锦山镇为PVY和辣椒轻斑驳病毒 (Pepper mild mottle virus, PMMoV);宁城县和喀喇沁旗西桥镇为BBWV2;复合侵染类型以2种病毒复合侵染为主,复合侵染率最高达61.54%。对测定的序列系统发育分析表明,赤峰地区4个辣椒产区的PMMoV和BBWV2存在遗传分化,PVY可能出现了新的遗传型。  结论  内蒙古赤峰地区不同辣椒主产区发生的病毒种类、检出率和病毒复合侵染种类均存在明显差异。
  • 图  1  sRNA长度分布

    Figure  1.  Distribution of sRNA length

    图  2  BBWV2 RNA依赖的RNA聚合酶基因部分核苷酸序列系统发育分析

    Figure  2.  Phylogenetic analysis on partial nucleotide sequence of RNA dependent RNA polymerase gene of BBWV2

    图  3  PMMoV CP基因部分核苷酸序列系统发育分析

    Figure  3.  Phylogenetic analysis on partial nucleotide sequence of CP gene of PMMoV

    图  4  PVY CP基因部分核苷酸序列系统发育分析

    Figure  4.  Phylogenetic analysis on partial nucleotide sequence of CP gene of PVY

    表  1  sRNA测序数据质量

    Table  1.   Quality of data obtained by sRNA

    样本
    编号
    Sample No.
    原始
    读数量
    Reads/M
    原始
    序列量
    Bases /M
    GC含量
    GC content/
    %
    质量值Q30/% 片段数量
    Contig
    number
    片段长
    度中值
    Contig N50/bp
    城子乡CZ 12.14 607 52.34 97.45 284 157
    锦山镇KQ 13.34 667 48.28 97.46 394 182
    宁城县NC 13.43 671 51.06 97.37 276 168
    西桥镇XQ 12.80 640 51.64 97.60 258 146
    Q30:Phred 数值大于 30 的碱基占总体碱基的百分比。
    Q30: ratio of bases of Phred number over 30 in total bases.
    下载: 导出CSV

    表  2  辣椒病毒检出率

    Table  2.   Positive viral detection rate on chili pepper plants

    采样地点
    Sampling site
    辣椒样本病毒检出率
    Rates of positive detection of viruses in pepper plants/%
    SPVG PMMoV BBWV2 PVY INLV SCMV
    城子乡CZ 0 61.54 92.31 0 23.08 0
    锦山镇KQ 0 58.33 41.67 83.33 0 0
    宁城县NC 0 25.00 58.33 0 0 0
    西桥镇XQ 0 20.00 30.00 0 0 0
    下载: 导出CSV

    表  3  辣椒病毒复合侵染率

    Table  3.   Occurrence rate of multiple viruses infection on chili pepper plants

    采样地点
    Sampling site
    病毒复合侵染种类
    Viruses of fused infection
    病毒检出率
    Rates of positive
    detection of viruses/%
    城子乡CZ BBWV2+PMMoV 61.54
    BBWV2+INLV 23.08
    BBWV2+INLV+PMMoV 23.08
    锦山镇KQ PMMoV+BBWV2 41.67
    PMMoV+PVY 58.33
    BBWV2+PVY 41.67
    PMMoV+BBWV2+PVY 25.00
    宁城县NC PMMoV+BBWV2 25.00
    西桥镇XQ PMMoV+BBWV2 15.38
    下载: 导出CSV
  • [1] 崔聪聪, 王秀芝, 张晓梅, 等. 赤峰市露地辣椒生产情况及经济效益调查分析 [J]. 现代农业科技, 2018, (24):92−93,95. doi: 10.3969/j.issn.1007-5739.2018.24.054

    CUI C C, WANG X Z, ZHANG X M, et al. Investigation and analysis on the production situation and economic benefit of pepper in Chifeng city [J]. Modern Agricultural Science and Technology, 2018(24): 92−93,95. (in Chinese) doi: 10.3969/j.issn.1007-5739.2018.24.054
    [2] 张晓梅, 王秀芝, 崔聪聪, 等. 2018年赤峰地区设施辣椒生产与市场情况分析 [J]. 中国蔬菜, 2019, (3):89−92.

    ZHANG X M, WANG X Z, CUI C C, et al. Production and market analysis of protected pepper in Chifeng area in 2018 [J]. China Vegetables, 2019(3): 89−92. (in Chinese)
    [3] 柴阿丽, 陈利达, 曹金强, 等. 利用siRNA高通量测序和RT-PCR技术鉴定引起茄子斑驳紫花病的病毒种类 [J]. 园艺学报, 2019, 46(3):508−518.

    CHAI A L, CHEN L D, CAO J Q, et al. Identification of viruses causing eggplant purple mottle flower disease by sirna high-throughput sequencing and RT-PCR detection [J]. Acta Horticulturae Sinica, 2019, 46(3): 508−518. (in Chinese)
    [4] 刘勇, 李凡, 李月月, 等. 侵染我国主要蔬菜作物的病毒种类、分布与发生趋势 [J]. 中国农业科学, 2019, 52(2):239−261. doi: 10.3864/j.issn.0578-1752.2019.02.005

    LIU Y, LI F, LI Y Y, et al. Identification, distribution and occurrence of viruses in the main vegetables of China [J]. Chinese Agricultural Sciences, 2019, 52(2): 239−261. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.02.005
    [5] SKELTON A, UZAYISENGA B, FOWKES A, et al. First report of Pepper veinal mottle virus, pepper yellows virus and a novel enamovirus in chilli pepper (Capsicum sp. ) in Rwanda [J]. New Disease Reports, 2018, 37(1): 5. doi: 10.5197/j.2044-0588.2018.037.005
    [6] 冯耿, 辛敏, 曹孟籍, 等. 深度测序发现贵阳发生的辣椒病毒病由多种病毒复合侵染所致 [J]. 植物病理学报, 2017, 47(5):591−597.

    FENG G, XIN M, CAO M J, et al. Identification of multiple viruses infecting hot pepper in Guiyang by deep sequencing [J]. Acta Phytopathologica Sinica, 2017, 47(5): 591−597. (in Chinese)
    [7] 廖震, 赵德刚, 赵懿琛. 利用小RNA深度测序技术检测分析小黄姜病毒 [J]. 基因组学与应用生物学, 2018, 37(6):2417−2422.

    LIAO Z, ZHAO D G, ZHAO Y C. Detection and analysis of viruses from small yellow ginger (Zingiber officinale Rosc. ) by small RNA deep sequencing technology [J]. Genomics and Applied Biology, 2018, 37(6): 2417−2422. (in Chinese)
    [8] 汤亚飞, 裴凡, 李正刚, 等. 基于小RNA深度测序技术鉴定侵染广东辣椒的病毒种类 [J]. 中国农业科学, 2019, 52(13):2256−2267. doi: 10.3864/j.issn.0578-1752.2019.13.006

    TANG Y F, PEI F, LI Z G, et al. Identification of viruses infecting peppers in Guangdong by small RNA deep sequencing [J]. Scientia Agricultura Sinica, 2019, 52(13): 2256−2267. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.13.006
    [9] 于海龙, 靳远, 刘婧, 等. 我国辣椒病毒病发生情况及发展趋势: 基于2018年和2019年辣椒主产区的调查 [J]. 中国蔬菜, 2020, (9):25−30.

    YU H L, JIN Y, LIU J, et al. Occurrence and development trend of pepper virus disease in China—based on main pepper producing areas investigation in 2018 and 2019 [J]. China Vegetables, 2020(9): 25−30. (in Chinese)
    [10] LANGMEAD B, TRAPNELL C, POP M, et al. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome [J]. Genome Biology, 2009, 10(3): R25. doi: 10.1186/gb-2009-10-3-r25
    [11] TAMURA K, STECHER G, KUMAR S. MEGA11: Molecular evolutionary genetics analysis version 11 [J]. Molecular Biology and Evolution, 2021, 38(7): 3022−3027. doi: 10.1093/molbev/msab120
    [12] VISSER M, BESTER R, BURGER J T, et al. Next-generation sequencing for virus detection: covering all the bases [J]. Virology Journal, 2016, 13: 85. doi: 10.1186/s12985-016-0539-x
    [13] SANTALA J, VALKONEN J P T. Sensitivity of small RNA-based detection of plant viruses [J]. Frontiers in Microbiology, 2018, 9: 939. doi: 10.3389/fmicb.2018.00939
    [14] GARCÍA-ARENAL F, FRAILE A, MALPICA J M. Variability and genetic structure of plant virus populations [J]. Annual Review of Phytopathology, 2001, 39: 157−186. doi: 10.1146/annurev.phyto.39.1.157
    [15] 李桑桑, 胡荣, 罗香文, 等. 湖北和广西辣椒脉斑驳病毒的检测及 遗传多样性分析 [J]. 南方农业学报, 2020, 51(7):1693−1698. doi: 10.3969/j.issn.2095-1191.2020.07.023

    LI S S, HU R, LUO X W, et al. Detection and genetic identity of Pepper veinal mottle virus in Hubei and Guangxi [J]. Journal of Southern Agriculture, 2020, 51(7): 1693−1698. (in Chinese) doi: 10.3969/j.issn.2095-1191.2020.07.023
    [16] 汤亚飞, 裴凡, 于琳, 等. 侵染广东辣椒的辣椒脉斑驳病毒的分子特征 [J]. 园艺学报, 2018, 45(11):2209−2216.

    TANG Y F, PEI F, YU L, et al. Molecular characterization of Chilli veinal mottle virus infecting pepper in Guangdong province [J]. Acta Horticulturae Sinica, 2018, 45(11): 2209−2216. (in Chinese)
    [17] PICARD C, DALLOT S, BRUNKER K, et al. Exploiting genetic information to trace plant virus dispersal in landscapes [J]. Annual Review of Phytopathology, 2017, 55: 139−160. doi: 10.1146/annurev-phyto-080516-035616
    [18] VASSILAKOS N, SIMON V, TZIMA A, et al. Genetic determinism and evolutionary reconstruction of a host jump in a plant virus [J]. Molecular Biology and Evolution, 2016, 33(2): 541−553. doi: 10.1093/molbev/msv222
    [19] FERRER R M, FERRIOL I, MORENO P, et al. Genetic variation and evolutionary analysis of broad bean wilt virus 2 [J]. Archives of Virology, 2011, 156(8): 1445−1450. doi: 10.1007/s00705-011-0990-3
    [20] GUAN X Y, YANG C X, FU J J, et al. Rapid evolutionary dynamics of pepper mild mottle virus [J]. Virus Research, 2018, 256: 96−99. doi: 10.1016/j.virusres.2018.08.006
    [21] GAO F L, ZOU W C, XIE L H, et al. Adaptive evolution and demographic history contribute to the divergent population genetic structure of Potato virus Y between China and Japan [J]. Evolutionary Applications, 2017, 10(4): 379−390. doi: 10.1111/eva.12459
    [22] 龚明霞, 赵虎, 王萌, 等. 广西辣椒病毒病调查及病原种类初步鉴定 [J]. 中国蔬菜, 2020, (4):74−79.

    GONG M X, ZHAO H, WANG M, et al. Investigation and preliminary identification of pathogeny species of pepper virus disease in Guangxi [J]. China Vegetables, 2020(4): 74−79. (in Chinese)
    [23] 郭思瑶, 童艳, 黄娅, 等. 重庆辣椒病毒病病原初步鉴定和分析 [J]. 园艺学报, 2015, 42(2):263−270.

    GUO S Y, TONG Y, HUANG Y, et al. Preliminary identification and analyses of viruses causing pepper virus disease in Chongqing, China [J]. Acta Horticulturae Sinica, 2015, 42(2): 263−270. (in Chinese)
    [24] 王少立, 谭玮萍, 杨园园, 等. 山东省辣椒主要病毒种类的分子检测与鉴定 [J]. 中国农业科学, 2017, 50(14):2728−2738. doi: 10.3864/j.issn.0578-1752.2017.14.009

    WANG S L, TAN W P, YANG Y Y, et al. Molecular detection and identification of main viruses on pepper in Shandong Province [J]. Scientia Agricultura Sinica, 2017, 50(14): 2728−2738. (in Chinese) doi: 10.3864/j.issn.0578-1752.2017.14.009
    [25] 姚玉荣, 陈国华, 冯兰香, 等. 北运蔬菜基地辣椒病毒病病原种类的分子检测 [J]. 中国蔬菜, 2013, (10):84−89. doi: 10.3969/j.issn.1000-6346.2013.10.014

    YAO Y R, CHEN G H, FENG L X, et al. Molecular detection of pepper viruses in southern vegetable production bases [J]. China Vegetables, 2013(10): 84−89. (in Chinese) doi: 10.3969/j.issn.1000-6346.2013.10.014
    [26] PEÑAFLOR M F G V, MAUCK K E, ALVES K J, et al. Effects of single and mixed infections of Bean pod mottle virus and Soybean mosaic virus on host-plant chemistry and host–vector interactions [J]. Functional Ecology, 2016, 30(10): 1648−1659. doi: 10.1111/1365-2435.12649
    [27] LAMICHHANE J R, VENTURI V. Synergisms between microbial pathogens in plant disease complexes: A growing trend [J]. Frontiers in Plant Science, 2015, 6: 385.
    [28] SAFARI M, ROOSSINCK M J. Coevolution of a persistent plant virus and its pepper hosts [J]. Molecular Plant-Microbe Interactions, 2018, 31(7): 766−776. doi: 10.1094/MPMI-12-17-0312-R
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  • 收稿日期:  2023-10-28
  • 修回日期:  2024-01-11
  • 网络出版日期:  2024-08-15
  • 刊出日期:  2024-07-28

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