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贵州不同生境半夏内生和根际菌群多样性及其与环境因子的相关性

陈晓芳 胡天骥 张翔宇 任锡毅 柳敏

陈晓芳,胡天骥,张翔宇,等. 贵州不同生境半夏内生和根际菌群多样性及其与环境因子的相关性 [J]. 福建农业学报,2024,39(7):826−838 doi: 10.19303/j.issn.1008-0384.2024.07.010
引用本文: 陈晓芳,胡天骥,张翔宇,等. 贵州不同生境半夏内生和根际菌群多样性及其与环境因子的相关性 [J]. 福建农业学报,2024,39(7):826−838 doi: 10.19303/j.issn.1008-0384.2024.07.010
CHEN X F, HU T J, ZHANG X Y, et al. Endophytic and Rhizosphere Microbes in Pinellia ternata and Habitat Soils in Guizhou Affected by Environmental Conditions [J]. Fujian Journal of Agricultural Sciences,2024,39(7):826−838 doi: 10.19303/j.issn.1008-0384.2024.07.010
Citation: CHEN X F, HU T J, ZHANG X Y, et al. Endophytic and Rhizosphere Microbes in Pinellia ternata and Habitat Soils in Guizhou Affected by Environmental Conditions [J]. Fujian Journal of Agricultural Sciences,2024,39(7):826−838 doi: 10.19303/j.issn.1008-0384.2024.07.010

贵州不同生境半夏内生和根际菌群多样性及其与环境因子的相关性

doi: 10.19303/j.issn.1008-0384.2024.07.010
基金项目: 贵州省中药材现代产业技术体系建设专项(GZCYTX2023-0204);贵州省人才基地项目(RCJD2020-21);毕节市揭榜挂帅项目(毕科合重大专项字〔2021〕3号);毕节市联合基金项目(毕科合yz〔2021〕5号);毕节市科技创新平台及人才团队项目(毕科合〔2023〕66号);毕节市优势中药材研发创新团队项目(毕委人领办通〔2023〕16号)
详细信息
    作者简介:

    陈晓芳(1986 —),女,硕士,副教授,主要从事特色资源分子生物学研究,E-mail:672409415@qq.com

    通讯作者:

    张翔宇(1986 —),男,硕士,研究员,主要从事药用植物资源工程研究,E-mail:304626335@qq.com

  • 中图分类号: S513

Endophytic and Rhizosphere Microbes in Pinellia ternata and Habitat Soils in Guizhou Affected by Environmental Conditions

  • 摘要:   目的  探究贵州不同生境半夏内生和根际土壤微生物群落多样性、物种组成、物种网络关系和功能菌群预测,并分析其与环境因子的相关性,为半夏菌群资源开发利用提供理论依据。  方法  对贵州野生半夏、规模化种植半夏和套种半夏的内生菌群、根际土壤菌群进行高通量测序,检测土壤理化因子和酶活性,并进行生物信息学分析。  结果  人工种植和野生半夏土壤环境因子差异性明显,且栽培方式对土壤理化性质与酶活有显著影响,人工种植样地的有机质含量高且土壤酸性强,野生样地酶活性偏高;半夏内生细菌优势菌属包括根瘤菌属(Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium)、假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、慢生根瘤菌属(Bradyrhizobium)等,半夏内生真菌优势菌属包括弯孢菌属(Campylospora)、新赤壳属(Neocosmospora)、丝核菌属(Rhizoctonia)、镰刀菌属(Fusarium)、Dactylonectria、炭角菌目(Xylariales)未分类属等,半夏根际优势菌属种类更丰富,各优势菌属在不同样本以不同丰度聚集且具有规律性,受生长环境和种植方式影响;细菌优势菌群中有10属、真菌优势菌群中有13属,与土壤理化性质或酶活性呈显著相关性,内生菌群对环境因子更为敏感,半夏内生菌群既互利又拮抗,根际土壤菌群网络关系相对稳定;半夏内生菌群优势菌属中除了未明确分类、未知菌属外,有益菌属丰度占比高。  结论  可通过人为调节土壤pH、施入菌肥等方式改良土壤微生态环境;半夏内生菌群包括根瘤菌、假单胞菌、芽孢杆菌、农研丝杆菌、木霉菌和绿僵菌等可开发利用,助力半夏栽培,促进半夏产业发展。
  • 图  1  不同生境半夏细菌OTU水平韦恩图

    Figure  1.  Venn plot of bacterial OTUs in P. ternata habitat soils

    图  2  不同生境半夏真菌OTU水平韦恩图

    Figure  2.  Venn plot of fungal OTUs in P. ternata habitat soils

    图  3  不同生境半夏细菌群落Shannon指数差异

    Figure  3.  Bacterial Shannon indexes of P. ternata habitat soils

    图  4  不同生境半夏真菌菌群Shannon指数差异

    * 表示差异显著(P ≤ 0.05) 。

    Figure  4.  Fungal Shannon indexes of P. ternata habitat soils

    * indicate significant difference ( P ≤ 0.05).

    图  5  不同生境半夏细菌OTU水平NMDS分析

    Figure  5.  NMDS analysis on bacterial OTUs of P. ternata habitat soils

    图  6  不同生境半夏真菌OTU水平NMDS分析

    Figure  6.  NMDS analysis on fungal OTUs of P. ternata habitat soils

    图  7  不同生境半夏细菌门水平物种组成

    Figure  7.  Bacteria species at phylum level in P. ternata habitat soils

    图  8  不同生境半夏真菌门水平物种组成

    Figure  8.  Fungi species at phylum level in P. ternata habitat soils

    图  9  不同生境半夏细菌属水平物种组成

    Figure  9.  Bacteria species at genus level in P. ternata habitat soils

    图  10  不同生境半夏真菌属水平物种组成

    Figure  10.  Fungi species at genus level in P. ternata habitat soils

    图  11  细菌属水平物种间相关性分析

    圆点的大小表示物种之间相关性强度,下同。

    Figure  11.  Correlations among bacteria species at genus level

    Size of dot indicates correlation between specie. Same for below.

    图  12  真菌属水平物种间相关性分析

    Figure  12.  Correlations among fungi species at genus level

    图  13  细菌潜在致病性物种贡献度

    Figure  13.  Contribution of potential pathogenic species

    图  14  细菌胁迫耐受物种贡献度

    Figure  14.  Contribution of stress-tolerant species

    图  15  真菌FUNGuild功能预测

    Figure  15.  FUNGuild-predicted functions of fungi

    图  16  不同生境半夏细菌属水平物种与环境因子相关性

    Figure  16.  Correlation between bacteria species and environmental factors in P. ternata habitat soils

    图  17  不同生境半夏真菌属水平物种与环境因子相关性

    Figure  17.  Correlation between fungi species at genus level and environmental factors in P. ternata habitat soils

    表  1  半夏采样信息

    Table  1.   Sampling of P. ternata

    编号
    Code
    采集地点
    Locality
    采集部位和对应编号
    Collection location and coding
    生长环境
    Growth environment
    经度
    Longitude/ (°)
    纬度
    Latitude/(°)
    海拔
    Altitude/m
    DH 毕节市大河乡 块茎和根须(DHK)、根际土(DHT) 林下野生 27.319420 104.920517 1638.5
    HZ 毕节市赫章县半夏种植基地 块茎和根须(HZK)、根际土(HZT) 大田人工起垄栽培 27.074467 104.420799 2216.4
    DF 毕节市百里杜鹃
    管委会鹏程管理区
    块茎和根须(DFK)、根际土(DFT) 滇重楼套种,人工起垄栽培,
    种植基地周边为针阔混交林
    27.232323 105.785965 1633.7
    下载: 导出CSV

    表  2  不同生境半夏土壤理化性质及酶活性

    Table  2.   Physiochemical properties and enzyme activities of P. ternata habitat soils

    指标
    Index
    野生DH 规模化种植HZ 套种DF
    总氮TN/(g·kg−1 1.45±0.01b 1.63±0.03a 1.42±0.02b
    总磷TP/(g·hg−1 0.08±0.01c 0.10±0.00b 0.21±0.00a
    总钾TK/(g·hg−1 1.45±0.03a 1.11±0.00b 1.01±0.02c
    有效氮AN/(mg·kg−1 207.73±4.68c 301.13±2.85a 234.86±0.43b
    有效钾AK/(mg·kg−1 462.84±1.08a 368.87±1.69c 398.78±1.35b
    有效磷AP/(mg·kg−1 44.23±0.44c 47.71±0.62b 76.82±0.42a
    有机质OM/(g·kg−1 44.64±0.78c 73.14±0.60a 55.33±0.23b
    pH 6.69±0.03b 5.45±0.02c 6.84±0.02a
    土壤脲酶 URE/(U·g−1 0.58±0.03b 0.83±0.08a 0.64±0.07b
    蔗糖酶 SC/(U·g−1 20.99±1.64a 10.47±0.43b 11.05±0.89b
    酸性磷酸酶 ACP/(U·g−1 2.35±0.06a 0.82±0.07c 1.52±0.10b
    多酚氧化酶 PPO/(U·g−1 3.80±0.13a 0.88±0.07c 1.79±0.03b
    同行中数值后面的不同小写字母表示处理间差异显著(P<0.05)。
    Data with different lowercase letters on same row indicate significant differences (P<0.05).
    下载: 导出CSV
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  • 收稿日期:  2023-11-01
  • 修回日期:  2024-04-30
  • 网络出版日期:  2024-08-15
  • 刊出日期:  2024-07-28

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