含ACC脱氨酶的根际促生菌对切花月季生长的影响

李文祥; 张天谣; 李洋; 李新艺; CAOMenghui; LIQinmei; 黄海泉; 黄美娟

含ACC脱氨酶的根际促生菌对切花月季生长的影响

西南林业大学园林园艺学院/国家林业和草原局西南风景园林工程技术研究中心/云南省功能性花卉资源及产业化技术工程研究中心/西南林业大学园林园艺花卉研发中心，云南　昆明，650224

基金项目: 国家林业和草原局推广项目（2020133126）；国家自然科学基金（31760001）；云南省自然科学基金(2011FB067)；云南省园林植物遗传改良与高效繁育博士生导师团队项目(503210103)

详细信息

作者简介:
李文祥（1995−），男，硕士研究生，研究方向：园林植物研究（E-mail：972403487@qq.com）

通讯作者:
黄海泉（1974−），男，教授，研究方向：园林植物研究（E-mail：haiquanl@163.com）

黄美娟（1972−），女，教授，研究方向：园林植物研究（E-mail：xmhhq2001@163.com）

中图分类号: S685.12
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出版历程
- 收稿日期: 2022-01-12
- 修回日期: 2023-03-29
- 网络出版日期: 2023-05-09

Effect of Rhizobacteria Containing ACC Deaminase on Growth of Rose Bush

College of Landscape Architecture and Horticulture Sciences/Southwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration)/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization/Research and Development Center of Landscape Plants and Horticulture Flowers, Southwest Forestry University, Kunming, Yunnan　650224, China

摘要

摘要: 目的探明含ACC脱氨酶的不同植物根际促生菌对切花月季白荔枝的促生效果。方法以白荔枝为试验材料，在其盆栽过程中施入含ACC脱氨酶的不同PGPR菌株，通过测定其株高、茎粗、分枝数等农艺性状、叶绿素a/b值、叶片保护酶活性及MDA含量、花朵乙烯释放量、光合及叶绿素荧光参数等生理指标，探讨不同菌株对白荔枝生长及生理的影响。结果施入含ACC脱氨酶的不同PGPR菌株对白荔枝生长及光合作用产生的影响有所差异，其中菌株F23和F195处理较对照株高增加25.9%和26.0%，且F23处理后茎粗比对照处理增加17.1%，菌株F23和F195处理叶绿素a/b也高于对照。菌株F23显著提高了根际土壤脲酶、磷酸酶和蔗糖酶活性至42.6%、 16.3%和48.8%；且菌株F23对POD以及CAT活性的提高有显著促进作用，分别达到对照的1.78和2.07倍，花朵乙烯释放量比对照减少37.0%。净光合速率经菌株F195和F23处理后显著高于对照，分别达对照的1.40倍和1.16倍。结论综合比较来看，菌株F23对切花月季白荔枝的促生效果最好。
- ACC脱氨酶 /
- 根际促生菌 /
- 切花月季 /
- 促生长 /
- 生理特性
Abstract: Objective Effect of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase on the productivity of rose bushes was studied. Method A pot experiment was conducted on White Lichee , a variety of roses commonly used commercially for cut flowers.Rhizobacteria carrying the plant growth promoter, ACC deaminase, were added to the soil, and effects on the agronomic traits, such as plant height, stem girth, and number of branches, as well as the physiological indexes, such as chlorophyll a/b, leaf protective enzyme, MDA content, flower ethylene release, photosynthesis, and chlorophyll fluorescence, of the plants monitored. Result In comparison with control, the incorporation of F23 or F195 PGPR, respectively, resulted in the rose bushes 25.9% and 26.0% taller with higher contents of chlorophyll a and chlorophyll b; F23, 17.1% larger stem girth, significantly greater activities of urease (by 42.6%), phosphatase (by 16.3%), and sucrase (by 48.8%) in rhizosphere soil, and POD (1.78x of control) and CAT (2.07x of control) in leaf. The floral ethylene release of the bushes treated with F23 was 37.0% lower than control, while the net photosynthetic rate of the F195-treated bushes significantly rose to 1.40 times and that of F23-treated bushes 1.16 times of control. Conclusion Overall, F23 appeared to most significantly promote the growth of White Lichee rose bushes among different PGPR tested.
- ACC deaminase /
- PGPR /
- rose /
- growth promotion /
- physiological characteristics

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图 1 含ACC脱氨酶的不同PGPR菌株对白荔枝叶片叶绿素a/b（A）及MDA含量（B）的影响

Figure 1. Effects of PGPR on leaf chlorophyll a/b (A) and MDA (B) of White Lichee

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图 2 含ACC脱氨酶的不同PGPR菌株对白荔枝花朵乙烯释放量及叶片抗氧化酶活性的影响

Figure 2. Effects of PGPR on floral ethylene production and leaf antioxidant enzyme activities of White Lichee

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表 1 含ACC脱氨酶的不同菌株信息

Table 1. Information on PGPR containing ACC deaminase

菌株编号 Strain number	属名 Genus	ACC脱氨酶活性 ACC deaminase activity/(U·mg⁻¹)
F195	Pseudomonas sp.	6.6910
F105	Stenotrophomonas sp.	0.9600
F11	Bacillus sp.	1.1342
F23	Arthrobacter sp.	1.0359
L11	Stenotrophomonas sp.	1.0356
L74	Stenotrophomonas sp.	1.0363
Z22	Bacillus sp.	1.0361

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表 2 含ACC脱氨酶的不同PGPR菌株对白荔枝主要农艺性状的影响

Table 2. Effects of PGPR on main agronomic characteristics of White Lichee

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter/mm	分枝数 Branch number	花径 Flower diameter/mm	花期 Flower period/d
F195	84.52±6.32 a	7.21±1.25 ab	1.33±0.14 a	129.68±11.67 a	15.33±2.08 a
F105	81.52±8.48 a	6.99±1.02 ab	1.67±0.63 a	119.06±10.89 a	13.00±1.00 ab
F11	87.66±2.46 a	7.59±1.35 ab	1.67±0.63 a	114.49±5.25 a	16.00±1.00 a
F23	84.47±6.89 a	8.02±1.53 a	1.42±0.38 a	118.94±2.90 a	15.33±2.08 a
L11	89.56±6.49 a	7.45±0.60 ab	1.42±0.14 a	116.95±15.21 a	13.33±2.31 ab
L74	84.47±10.25 a	7.16±1.23 ab	1.75±0.25 a	114.71±9.70 a	13.67±1.53 ab
Z22	89.12±5.77 a	6.97±0.99 ab	1.75±0.00 a	122.06±5.21 a	11.67±1.16 b
Mix	80.85±5.44 a	7.20±1.39 ab	1.58±0.29 a	121.12±4.27 a	13.33±1.53 ab
CK	67.10±1.52 b	6.85±1.44 b	2.00±0.25 a	117.42±0.87 a	14.33±0.58 ab
表内数据表示平均值±标准差，同列不同小写字母表示差异显著(P＜0.05)，3个生物学重复。下同。 Data are mean ± SD; those with different lowercase letters on same column indicate significant differences on biological triplicates (P＜0.05).Same for below.

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表 3 含ACC脱氨酶的不同PGPR菌株对白荔枝根际土壤酶活的影响

Table 3. Effect of PGPR on soil enzyme activity in rhizosphere of White Lichee

处理 Treatment	脲酶活性 Urease activity/ (mg·g⁻¹·d⁻¹)	磷酸酶活性 Phosphatase activity/ (mg·g⁻¹·d⁻¹)	蔗糖酶活性 Sucrase activity/ (mg·g⁻¹·d⁻¹)
F195	0.355±0.011 a	1.035±0.011 a	22.474±0.820 b
F105	0.278±0.030 c	0.764±0.007 e	12.392±1.325 c
F11	0.229±0.016 d	0.993±0.008 b	12.458±0.594 c
F23	0.338±0.015 ab	1.041±0.007 a	33.475±2.000 a
L11	0.295±0.032 c	0.886±0.010 c	11.374±0.802 cd
L74	0.308±0.011 bc	0.991±0.011 b	9.863±0.205 d
Z22	0.237±0.012 d	0.884±0.001 c	6.809±1.312 e
Mix	0.277±0.023 c	0.790±0.005 d	7.006±1.035 e
CK	0.237±0.012 d	0.895±0.010 c	7.433±1.133 e

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表 4 含ACC脱氨酶的不同PGPR菌株对白荔枝光合特性的影响

Table 4. Effect of PGPR on photosynthesis of White Lichee

处理 Treatment	净光合速率 P_n/(μmol·m⁻²·s⁻¹)	气孔导度 G_s/(mol·m⁻²·s⁻¹)	胞间CO₂浓度 C_i/(μmol·mol⁻¹)	蒸腾速率 T_r/(mmol·m⁻²·s⁻¹)	水分利用效率 P_n/T_r
F195	9.34±0.45 a	0.278±0.094 a	427±33.78 a	3.19±1.19 a	3.18±1.07 a
F105	6.32±0.14 d	0.084±0.034 c	362±21.17 bc	1.90±0.83 a	3.68±1.23 a
F11	7.11±0.09 c	0.077±0.023 c	363±6.43 bc	1.77±0.61 a	4.39±1.62 a
F23	7.79±0.35 b	0.120±0.020 bc	387±18.25 abc	2.18±0.82 a	3.94±1.57 a
L11	6.97±0.28 c	0.093±0.032 c	376±54.60 abc	2.38±1.81 a	4.01±2.17 a
L74	7.98±0.16 b	0.181±0.063 b	404±17.62 abc	3.64±1.27 a	2.41±0.96 a
Z22	6.84±0.22 c	0.089±0.016 c	353±15.04 c	1.83±0.56 a	3.96±1.06 a
Mix	7.81±0.34 b	0.135±0.036 bc	411±25.54 bc	2.24±0.96 a	3.84±1.27 a
CK	6.69±0.09 cd	0.106±0.035 bc	389±25.06 abc	1.81±0.58 a	4.02±1.52 a

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表 5 含ACC脱氨酶的不同PGPR菌株对白荔枝叶绿素荧光特性的影响

Table 5. Effect of PGPR on chlorophyll fluorescence of White Lichee

处理 Treatment	最大光化学效率 F_v/F_m	实际光化学效率 Φ_PSII	光化学淬灭系数 qP	非光化学淬灭系数 qN	表观光合电子传递效率ETR
F195	0.787±0.019 a	0.566±0.029 d	0.293±0.067 a	0.805±0.054 a	3.073±0.416 c
F105	0.789±0.028 a	0.692±0.021 ab	0.324±0.056 a	0.726±0.081 a	7.953±2.790 ab
F11	0.777±0.017 a	0.618±0.064 cd	0.251±0.052 a	0.782±0.031 a	4.880±1.750 bc
F23	0.800±0.021 a	0.709±0.027 ab	0.311±0.024 a	0.812±0.040 a	6.147±1.684 abc
L11	0.776±0.015 a	0.667±0.037 bc	0.339±0.022 a	0.783±0.030 a	5.640±0.737 bc
L74	0.780±0.019 a	0.659±0.019 bc	0.348±0.061 a	0.797±0.090 a	5.567±1.362 bc
Z22	0.780±0.020 a	0.642±0.067 bc	0.304±0.076 a	0.781±0.057 a	3.867±0.167 c
Mix	0.808±0.013 a	0.740±0.025 a	0.343±0.046 a	0.788±0.067 a	9.353±2.136 a
CK	0.794±0.001 a	0.420±0.024 e	0.330±0.045 a	0.721±0.064 a	7.900±3.251 ab

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