Cloning and Sequence Analysis of Myostatin(MSTN) Gene from Fujian White Rabbit

In order to master the molecular basis of Myostatin(MSTN) gene that negatively regulates skeletal muscle growth and development in Fujian white rabbit, Fujian white rabbit MSTN gene cDNA was amplified and cloned from total RNA of muscle by RT-PCR and RACE(rapid amplification of cDNA ends). Sequence analysis indicated that the full cDNA (1 551 bp, Gen Bank accession number:KX084386) consisted of a 5'-terminal untranslated region (UTR) (136 bp), a 3'-terminal UTR (287 bp) and an ORF(1 128 bp). The open reading frame encoded a deduced protein with 375 amino acids residues. There were one TGF-beta propeptide superfamily domains at amino acids encoded by the 97 to 768 nucleotides and one TGF-beta family domains at amino acids encoded by the 841 to 1 125 nucleotides. Comparison of Fujian white rabbit MSTN with those of other rabbit, human, pig, mouse, horse, cattle, sheep, deer, baboons, orangutan and monkey showed that the amino acids horno1ogy were 99.9%, 92.8%, 94.4%, 86.9%, 95.2%, 89.1%, 91.0%, 90.3%, 95.9%, 96.4%, 95.9%, respectively. It provides the basic data for further research on structure function, expression regulation and molecular breeding of MSTN gene in Fujian white rabbit.