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YUAN B, KE L N, LIAN Y P, et al. Changes of Microbial Community in Agaricus bisporus Culture Substrate during Fermentation [J]. Fujian Journal of Agricultural Sciences,2022,37(9):1246−1253 doi: 10.19303/j.issn.1008-0384.2022.009.018
Citation: YUAN B, KE L N, LIAN Y P, et al. Changes of Microbial Community in Agaricus bisporus Culture Substrate during Fermentation [J]. Fujian Journal of Agricultural Sciences,2022,37(9):1246−1253 doi: 10.19303/j.issn.1008-0384.2022.009.018

Changes of Microbial Community in Agaricus bisporus Culture Substrate during Fermentation

doi: 10.19303/j.issn.1008-0384.2022.009.018
  • Received Date: 2022-04-02
  • Rev Recd Date: 2022-06-20
  • Available Online: 2022-10-05
  •   Objective  Changes of the microbial community in fermentation of substrates for cultivating Agaricus bisporus were investigated.   Method  The microbial community characteristics of a compound A. bisporus culture substrates containing spent Flammulina velutiper and Pleurotus eryngii materials were monitored during the 7 stages, Ag1 to Ag7 from pile building to 1st and 2nd fermentation, using the high throughput 16S rDNA full-length sequencing of Pacbio platform.   Result  A total of 715 OTUs were obtained in the fermentation process (i.e., 328, 340, 294, 377, 364, 166, and 174 for each stage) with 161 OTUs commonly found in all stage. The microbes included 21 phyla, 299 genera, and 399 species. At phylum level, Fimicutes, Proteobacteria, Bacteroidetes, and Gemmatimonadetes had higher abundance in all 7 stages. The dominant genus in the substrate during the pile building and 1st fermentation stages was Ureibacillus, while Limnochordaceae, S0134_terrestrial_group, Thermobacillu, and Ruminiclostridium stood out in the 2nd fermentation stage. At species level, Ureibacillus thermophilus and Ureibacillus terrenus were dominant in the pile building and 1st fermentation stages. In the 2nd fermentation stage, the species in the genus of Limnochordaceae were the greatest in relative abundance. It appeared that the microbial abundance and diversity increased gradually before the onset of 2nd fermentation. The community structure of the substrate varied significantly between the 1st and the 2nd fermentation, but the deviation diminished significantly after the 2nd fermentation leaving mostly the dominant species that basically functioned to degrade the waste mushroom material enriching the fertilization effect.   Conclusion  The full-length sequencing technology clearly identified the dominant species unveiling many previously unclassified microorganisms. The results helped to better optimize the substrate fermentation process for an improved cultivation of A. bisporus.
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