Molecular Mechanism of <i>traf6 like</i> Gene in Innate Immunity of <i>Procambarus clarkii</i>

LIN Sihan; YANG Bingbing; LI Qianqian; SHEN Xiuli; DU Zhiqiang

doi:10.19303/j.issn.1008-0384.2022.010.001

LIN S H, YANG B B, LI Q Q, et al. Molecular Mechanism of traf6 like Gene in Innate Immunity of Procambarus clarkii J. Fujian Journal of Agricultural Sciences，2022，37（10）：1245−1249. DOI: 10.19303/j.issn.1008-0384.2022.010.001

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Molecular Mechanism of traf6 like Gene in Innate Immunity of Procambarus clarkii

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Abstract

Objective Role of traf6 like gene in innate immunity regulation of Procambarus clarkia(Pc-traf6 like) was investigated.
Methods RNAi technology was employed to interfere the expression of Pc-traf6 like gene. Bacterial simulation was performed to determine the changes of apoptosis-related gene expression by real-time quantitative PCR (qRT-PCR).
Result The interference on the gene was effective after 48 h for the subsequent experiments. The interference-induced pro-apoptotic gene Bax, anti-apoptotic gene Bcl 2, and apoptotic protease Caspase 3 were upregulated in varying degrees with the stimulation of Staphylococcus aureus or Edwardsiella ictaluri.
Conclusion After the Pc-traf6 like gene was effectively interfered, the relative expressions of Bax, Bcl 2, and Caspase 3 were upregulated after the P. clarkia was stimulated by either S. aureus or E. ictaluri indicating an involvement of Pc-traf6 like gene in the innate immune response by the regulating apoptosis.

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Molecular Mechanism of traf6 like Gene in Innate Immunity of Procambarus clarkii

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