Establishment of a Triple-primer Multiplex PCR Assay for Discriminative Detection of Duck and Goose Circoviruses

WANG Mengge; HUANG Yu; FU Qiuling; CHENG Longfei; WAN Chunhe; LIU Rongchang; CHEN Hongmei; JIANG Nansong; LIANG Qizhang; SHI Shaohua; LIN Jiansheng; FU Guanghua

doi:10.19303/j.issn.1008-0384.2023.01.002

WANG M G, HUANG Y, FU Q L, et al. Establishment of a Triple-primer Multiplex PCR Assay for Discriminative Detection of Duck and Goose Circoviruses [J]. Fujian Journal of Agricultural Sciences，2023，38（1）：7−11. DOI: 10.19303/j.issn.1008-0384.2023.01.002

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Establishment of a Triple-primer Multiplex PCR Assay for Discriminative Detection of Duck and Goose Circoviruses

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Objective A PCR assay was established for discriminative detection of duck circovirus (DuCV) and goose circovirus (GoCV).
Methods Three primers designed according to the genome sequences of DuCV and GoCV deposited in GenBank database were used for the development of a Triple-primer Multiplex PCR (tpm-PCR).
Results The established assay could specifically identify DuCV and GoCV by the amplified fragments within the detection limits of 110 pg·μL⁻¹ and 80 pg·μL⁻¹, respectively. The methodology was verified with the test results by PCR for the individual virus in clinical cases.
Conclusion The tpm-PCR assay was specific and sensitive as a discriminative detection method between DuCV and GoCV. It facilitated the clinical diagnosis and epidemiological monitoring on the circoviral cases in waterfowls.

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Establishment of a Triple-primer Multiplex PCR Assay for Discriminative Detection of Duck and Goose Circoviruses

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