Morphological and SRAP Markers-based Genetic Diversity Determination on Phaseolus lunatus L. Germplasms

  Objective  Genetic diversity and relationship of Phaseolus lunatus L. germplasms were determined using the morphological and SRAP markers.

  Method  Twenty-six quantitative and 18 quality traits of 22 lima bean germplasms were analyzed based on the morphological as well as the SRAP molecular markers.

  Result  Twenty-eight pairs of SRAP primers were selected to obtain 158 amplified polymorphic bands with an average polymorphism ratio of 77.75%. The two methods applying different markers clustered the 22 varieties into 3 categories. Genetically, Shanghengshan10-2-6 and Xiahengshan 10-3-3 were relatively close. They might have gone through numerous gene exchanges in the past.

  Conclusion  The 22 lima bean germplasms were rich in genetic diversity. The morphological and SRAP molecular markers-based clustering essentially agreed with the classification by the pod size.