Objective SNP markers development and genetic diversity analysis of Canarium album were perfomed to facilitate the protection and utilization of germplasm resources of C. album.
Method SNP markers of C. album were developed based on SLAF-Seq technology, and the genetic structure and genetic diversity were studied by phylogenetic analysis, population cluster analysis and principal component analysis.
Result A total of 506 701 SLAF loci were mined, including 27 108 polymorphic SLAF loci, and 361 386 SNP markers were developed. Based on these SNP markers, germplasm resources of C. album were respectively divided into 3 and 6 groups by phylogenetic tree and population cluster analysis. The overall Nei diversity index and Shanon Wiener index were 0.321 and 0.472, respectively. The results of two classification methods showed that germplasm resources of C. album from different regions were not classified strictly according to regional distribution.
Conclusion The genetic diversity of germplasm resources of C. album had great genetic diversity which were exchange frequently among different regions, and the developed SNP markers of C. album could effectively identify the germplasm resources.
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