Cloning and Expression of <i>MeERF1.2</i> in Cassava

ZENG Jian; WU Weishan; XIE Caihong; SHEN Zixin; YE Xiaoxue; YAN Yan; LI Bing; CHEN Zhisheng; PENG Hongyuan; HU Wei; ZENG Liwang

doi:10.19303/j.issn.1008-0384.2023.04.003

Volume 38 Issue 4

Apr. 2023

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 410-416

ZENG J, WU W S, XIE C H, et al. Cloning and Expression of MeERF1.2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：410−416 doi: 10.19303/j.issn.1008-0384.2023.04.003

Citation:

ZENG J, WU W S, XIE C H, et al. Cloning and Expression of MeERF1.2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：410−416 doi: 10.19303/j.issn.1008-0384.2023.04.003

ZENG J, WU W S, XIE C H, et al. Cloning and Expression of MeERF1.2 in Cassava [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：410−416 doi: 10.19303/j.issn.1008-0384.2023.04.003

Citation:

PDF( 1087 KB)

Cloning and Expression of MeERF1.2 in Cassava

doi: 10.19303/j.issn.1008-0384.2023.04.003

ZENG Jian^1
,,
WU Weishan¹,
XIE Caihong¹,
SHEN Zixin¹,
YE Xiaoxue²,
YAN Yan^{2, 3},
LI Bing⁴,
CHEN Zhisheng²,
PENG Hongyuan¹,
HU Wei^{2, 3},
ZENG Liwang^{2, 3
,
,}

1.
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region/Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan, Guangdong 　512005, China
2.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science,　Haikou, Hainan 571101, China
3.
Institute of Scientific and Technical Information, Chinese Academy of Tropical Agricultural Science, Haikou, Hainan 　571101, China
4.
The First Middle School of Pingjiang Country, Yueyang, Hunan　414500, China

Received Date: 2022-06-09
Accepted Date: 2022-06-09
Rev Recd Date: 2022-11-15

Available Online: 2023-03-28

Publish Date: 2023-04-28

Abstract

Abstract

Objective Critical factors in the ethylene signal transduction pathway involving the post-harvest physiological deterioration (PPD) of cassava were investigated. Method One of the possible ethylene response factors (ERFs) in Manihot esculenta cv.SC8, MeERF1.2, was cloned using RT-PCR to analyze the expressions during PPD. Physicochemical properties, conserved domain, genetic evolutionary relationship, and protein structure of the gene were studied with bioinformatics tools. Distribution of MeERF1.2 in the plant cells was verified by online software and subcellular localization. Result MeERF1.2 had an ORF of 660 bp encoded 188 amino acids with a molecular weight of 25.04 kD and a pI of 5.61. The protein contained an AP2 domain showing a high sequence similarity of 88.74% with the HbERF1B-like gene. It was localized in the nucleus and significantly upregulated during PPD from 0 to 6, 12, and 48 h displaying an apparent induction trend in the process. Conclusion MeERF1.2 was one of the ERFs induced in cassava tubers during PPD and played a vital role in the signal transduction pathway of the plant.
- Cassava,
- ethylene,
- ERF,
- ROS,
- post-harvest physiological deterioration

FullText(HTML)

References(32)

References

[1]	张鹏, 杨俊, 周文智, 等. 能源木薯高淀粉抗逆分子育种研究进展与展望 [J]. 生命科学, 2014, 26(5):465−473. ZHANG P, YANG J, ZHOU W Z, et al. Progress and perspective of cassava molecular breeding for bioenergy development [J]. Chinese Bulletin of Life Sciences, 2014, 26(5): 465−473.(in Chinese)
[2]	HU W, KONG H, GUO Y L, et al. Comparative physiological and transcriptomic analyses reveal the actions of melatonin in the delay of postharvest physiological deterioration of cassava [J]. Frontiers in Plant Science, 2016, 7: 736.
[3]	郭靖, 章玉香, 黄芷颐, 等. 木薯MePYL12基因克隆及采后生理性变质过程的表达分析 [J]. 福建农业学报, 2021, 36(1):17−23. GUO J, ZHANG Y X, HUANG Z Y, et al. Cloning and post-harvest physiological deterioration expression of MePYL12 of cassava [J]. Fujian Journal of Agricultural Sciences, 2021, 36(1): 17−23.(in Chinese)
[4]	WESTBY A. Cassava utilization, storage and small-scale processing[M]//Cassava: biology, production and utilization. UK: CABI Publishing, 2001: 281-300.
[5]	马秋香, 许佳, 乔爱民, 等. 木薯储藏根采后生理性变质研究进展 [J]. 热带亚热带植物学报, 2009, 17(3):309−314. MA Q X, XU J, QIAO A M, et al. Current progress in studies on post-harvest physiological deterioration of cassava storage roots [J]. Journal of Tropical and Subtropical Botany, 2009, 17(3): 309−314.(in Chinese)
[6]	IYER S, MATTINSON D S, FELLMAN J K. Study of the early events leading to cassava root postharvest deterioration [J]. Tropical Plant Biology, 2010, 3(3): 151−165. doi: 10.1007/s12042-010-9052-3
[7]	HERSHKOVITZ V, FRIEDMAN H, GOLDSCHMIDT E E, et al. Induction of ethylene in avocado fruit in response to chilling stress on tree [J]. Journal of Plant Physiology, 2009, 166(17): 1855−1862. doi: 10.1016/j.jplph.2009.05.012
[8]	赵赫, 陈受宜, 张劲松. 乙烯信号转导与植物非生物胁迫反应调控研究进展 [J]. 生物技术通报, 2016, 32(10):1−10. ZHAO H, CHEN S Y, ZHANG J S. Ethylene signaling pathway in regulating plant response to abiotic stress [J]. Biotechnology Bulletin, 2016, 32(10): 1−10.(in Chinese)
[9]	施怡婷, 杨淑华. 中国科学家在乙烯信号转导领域取得突破性进展 [J]. 植物学报, 2016, 51(3):287−289. SHI Y T, YANG S H. Chinese scientists made breakthrough in study on ethylene signaling transduction in plants [J]. Chinese Bulletin of Botany, 2016, 51(3): 287−289.(in Chinese)
[10]	REN M Y, FENG R J, SHI H R, et al. Expression patterns of members of the ethylene signaling-related gene families in response to dehydration stresses in cassava [J]. PLoS One, 2017, 12(5): e0177621. doi: 10.1371/journal.pone.0177621
[11]	CAsO W H, LIU J, CHEN T, et al. Ethylene receptor signaling and plant salt-stress responses[C]//RAMINA A, CHANG C, GIOVANNONI J, et al. Advances in Plant Ethylene Research. Dordrecht: Springer, 2007: 333-339.
[12]	于延文, 黄荣峰. 乙烯与植物抗逆性 [J]. 中国农业科技导报, 2013, 15(2):70−75. YU Y W, HUANG R F. Ethylene and plant resistance to adversity [J]. Journal of Agricultural Science and Technology, 2013, 15(2): 70−75.(in Chinese)
[13]	DESIKAN R, LAST K, HARRETT-WILLIAMS R, et al. Ethylene-induced stomatal closure in Arabidopsis occurs via AtrbohF-mediated hydrogen peroxide synthesis [J]. The Plant Journal:for Cell and Molecular Biology, 2006, 47(6): 907−916. doi: 10.1111/j.1365-313X.2006.02842.x
[14]	杨洋. 拟南芥乙烯合成因子依赖ROS代谢调节盐胁迫反应[D]. 开封: 河南大学, 2011. YANG Y. The ethylene biosynthesis factors mediate Arabidopsis tolerance to NaCl stress in dependence on ROS metabolism[D]. Kaifeng: Henan University, 2011. (in Chinese)
[15]	MA Y R, YANG M N, WANG J J, et al. Application of exogenous ethylene inhibits postharvest peel browning of ‘Huangguan’ pear [J]. Frontiers in Plant Science, 2017, 7: 2029.
[16]	BLEECKER A B, KENDE H. Ethylene: A gaseous signal molecule in plants [J]. Annual Review of Cell and Developmental Biology, 2000, 16: 1−18. doi: 10.1146/annurev.cellbio.16.1.1
[17]	ALONSO J M, STEPANOVA A N, SOLANO R, et al. Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis [J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(5): 2992−2997. doi: 10.1073/pnas.0438070100
[18]	ZHAO Q, GUO H W. Paradigms and paradox in the ethylene signaling pathway and interaction network [J]. Molecular Plant, 2011, 4(4): 626−634. doi: 10.1093/mp/ssr042
[19]	史庆玲, 李忠峰, 董永彬, 等. 植物乙烯信号转导通路及其相关基因的研究进展 [J]. 生物技术进展, 2019, 9(5):449−454. SHI Q L, LI Z F, DONG Y B, et al. Progress on ethylene signal transduction pathway and related genes in plants [J]. Current Biotechnology, 2019, 9(5): 449−454.(in Chinese)
[20]	葛宝宇, 林轶, 侯和胜. ERF类转录因子的结构与功能 [J]. 安徽农学通报, 2007, 13(20):32−35. doi: 10.3969/j.issn.1007-7731.2007.20.013 GE B Y, LIN Y, HOU H S. Structure and function of ERF transcription factors [J]. Anhui Agricultural Science Bulletin, 2007, 13(20): 32−35.(in Chinese) doi: 10.3969/j.issn.1007-7731.2007.20.013
[21]	NAKANO T, SUZUKI K, FUJIMURA T, et al. Genome-wide analysis of the ERF gene family in Arabidopsis and rice [J]. Plant Physiology, 2006, 140(2): 411−432. doi: 10.1104/pp.105.073783
[22]	SHOJI T, MISHIMA M, HASHIMOTO T. Divergent dna-binding specificities of a group of ethylene response factor transcription factors involved in plant defense [J]. Plant Physiology, 2013, 162(2): 977−990. doi: 10.1104/pp.113.217455
[23]	ABIRI R, SHAHARUDDIN N A, MAZIAH M, et al. Role of ethylene and the APETALA 2/ethylene response factor superfamily in rice under various abiotic and biotic stress conditions [J]. Environmental and Experimental Botany, 2017, 134: 33−44. doi: 10.1016/j.envexpbot.2016.10.015
[24]	SOLANO R, STEPANOVA A, CHAO Q, et al. Nuclear events in ethylene signaling: A transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1 [J]. Genes & Development, 1998, 12(23): 3703−3714.
[25]	QIN F, SAKUMA Y, LI J, et al. Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L [J]. Plant and Cell Physiology, 2004, 45(8): 1042−1052. doi: 10.1093/pcp/pch118
[26]	VANDERSCHUREN H, NYABOGA E, POON J S, et al. Large-scale proteomics of the cassava storage root and identification of a target gene to reduce postharvest deterioration [J]. The Plant Cell, 2014, 26(5): 1913−1924. doi: 10.1105/tpc.114.123927
[27]	张云川, 曹天澳, 雷骥良, 等. 橡胶草ERF3基因的克隆、亚细胞定位及表达分析 [J]. 植物生理学报, 2021, 57(6):1261−1270. ZHANG Y C, CAO T A, LEI J L, et al. Cloning, subcellular localization and expression analysis of TkERF₃ in Taraxacum kok-saghyz [J]. Plant Physiology Journal, 2021, 57(6): 1261−1270.(in Chinese)
[28]	JUNG H, CHUNG P J, PARK S H, et al. Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance [J]. Plant Biotechnology Journal, 2017, 15(10): 1295−1308. doi: 10.1111/pbi.12716
[29]	LEE D K, YOON S, KIM Y S, et al. Rice OsERF71-mediated root modification affects shoot drought tolerance [J]. Plant Signaling & Behavior, 2017, 12(1): e1268311.
[30]	ZHANG G Y, CHEN M, LI L C, et al. Overexpression of the soybean GmERF₃ gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco [J]. Journal of Experimental Botany, 2009, 60(13): 3781−3796. doi: 10.1093/jxb/erp214
[31]	CHARFEDDINE M, CHARFEDDINE S, GHAZALA I, et al. Investigation of the response to salinity of transgenic potato plants overexpressing the transcription factor StERF₉₄ [J]. Journal of Biosciences, 2019, 44(6): 141. doi: 10.1007/s12038-019-9959-2
[32]	SHIN S Y, PARK M H, CHOI J W, et al. Gene network underlying the response of harvested pepper to chilling stress [J]. Journal of Plant Physiology, 2017, 219: 112−122. doi: 10.1016/j.jplph.2017.10.002