Response Surface Optimization on Enzymatic Extraction Process of Turmeric Starch

CHI Hongfei; FU Lijuan; SI Ruiru; WEI Hang; HUANG Biao; ZENG Shaoxiao; FU Jianwei

doi:10.19303/j.issn.1008-0384.2023.04.015

Volume 38 Issue 4

Apr. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 506-514

CHI H F, FU L J, SI R R, et al. Response Surface Optimization on Enzymatic Extraction Process of Turmeric Starch [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：506−514 doi: 10.19303/j.issn.1008-0384.2023.04.015

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CHI H F, FU L J, SI R R, et al. Response Surface Optimization on Enzymatic Extraction Process of Turmeric Starch [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：506−514 doi: 10.19303/j.issn.1008-0384.2023.04.015

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Response Surface Optimization on Enzymatic Extraction Process of Turmeric Starch

doi: 10.19303/j.issn.1008-0384.2023.04.015

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Institute of Quality Standards & Testing Technology for Agro-products, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-Products Quality & Safety, Fuzhou, Fujian 350003, China

Received Date: 2023-02-09
Rev Recd Date: 2023-03-25

Available Online: 2023-05-24

Publish Date: 2023-04-28

Abstract

Abstract

Objective Optimization on the enzymatic extraction process of starch from turmeric was improved. Method Using fresh turmeric as raw material, the best enzyme was screened among several proteases according to the starch extraction rate. A single-factor test was conducted to define the range of the time, digestion temperature, pH and the amount of neutral protease for extraction. Based on the single-factor test results, a 4-factor, 3-level Box-Behnken response surface optimization experiment was designed on 29 treatments with 3 replicates each to determine the optimal processing conditions. Results The optimized process applied 0.13% added neutral protease in a solution at pH 6.83 held at 51.45 °C for 6.20 h to achieve 62.00% turmeric starch extraction. For practical application the conditions were modified to be pH 6.8, 52°C, and 6 h to obtain a turmeric extraction rate of 60.42%. The experimental results fitted well with the prediction model. The fitted model was in good agreement with the actual one. Conclusion The enzymatic extraction of turmeric starch was optimized by response surface methodology in preparation for industrialized production.
- Turmeric,
- starch,
- extraction,
- response surface methodology,
- neutral protease

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References(31)

References

[1]	仉瑜, 张洪兵, 郭虹, 等. 姜黄的研究进展及质量标志物(Q-Marker)的预测分析 [J]. 中草药, 2021, 52(15):4700−4710. doi: 10.7501/j.issn.0253-2670.2021.15.029 ZHANG Y, ZHANG H B, GUO H, et al. Research progress on Curcumae Longae Rhizoma and predictive analysis of its quality markers [J]. Chinese Traditional and Herbal Drugs, 2021, 52(15): 4700−4710.(in Chinese) doi: 10.7501/j.issn.0253-2670.2021.15.029
[2]	庞新华, 何新华, 周全光, 等. 姜黄种质资源的保存及其主要性状评价 [J]. 热带作物学报, 2014, 35(6):1047−1055. doi: 10.3969/j.issn.1000-2561.2014.06.002 PANG X H, HE X H, ZHOU Q G, et al. Conservation of Curcuma germplasm resources and evaluation of their main characters [J]. Chinese Journal of Tropical Crops, 2014, 35(6): 1047−1055.(in Chinese) doi: 10.3969/j.issn.1000-2561.2014.06.002
[3]	田芳, 柴玉荣, 江亚南, 等. 姜黄素通过下调IκBα磷酸化抑制食管鳞癌细胞的体外增殖 [J]. 基础医学与临床, 2011, 31(7):767−772. TIAN F, CHAI Y R, JIANG Y N, et al. The effect of curcumin through suppression of IκBα phosphorylation on inhibition of proliferation of esophageal squamous cell carcinoma cell lines [J]. Basic & Clinical Medicine, 2011, 31(7): 767−772.(in Chinese)
[4]	周欣, 李章万, 王道平, 等. 姜科姜黄属植物有效成分的研究 [J]. 分析测试学报, 2004, 23(6):53−56. doi: 10.3969/j.issn.1004-4957.2004.06.015 ZHOU X, LI Z W, WANG D P, et al. Effective chemical constituents in essential oils from plants of Curcuma genus [J]. Journal of Instrumental Analysis, 2004, 23(6): 53−56.(in Chinese) doi: 10.3969/j.issn.1004-4957.2004.06.015
[5]	国家药典委员会. 中华人民共和国药典-三部: 2005年版[M]. 北京: 化学工业出版社, 2005.
[6]	汤敏燕, 汪洪武, 孙凌峰. 中药姜黄挥发油化学成分研究 [J]. 江西师范大学学报(自然科学版), 2000, 24(3):274−277. doi: 10.16357/j.cnki.issn1000-5862.2000.03.018 TANG M Y, WANG H W, SUN L F. Studies on the chemical constituents of essential oil from Chinese traditional drug Jianghuang(tubers of Curcuma) [J]. Journal of Jiangxi Normal University (Natural Sciences Edition), 2000, 24(3): 274−277.(in Chinese) doi: 10.16357/j.cnki.issn1000-5862.2000.03.018
[7]	MIYAZAKI T, WADA M, KAWAHARA H, et al. Dynamic load at baseline can predict radiographic disease progression in medial compartment knee osteoarthritis [J]. Annals of the Rheumatic Diseases, 2002, 61(7): 617−622. doi: 10.1136/ard.61.7.617
[8]	LIN X Y, BAI D P, WEI Z X, et al. Curcumin attenuates oxidative stress in RAW_264.7 cells by increasing the activity of antioxidant enzymes and activating the Nrf2-Keap1 pathway [J]. PLoS One, 2019, 14(5): e0216711. doi: 10.1371/journal.pone.0216711
[9]	LIU Y, LIU Y H, HUANG X C, et al. Protective effects and mechanism of curcumin on myocardial injury induced by coronary microembolization [J]. Journal of Cellular Biochemistry, 2019, 120(4): 5695−5703. doi: 10.1002/jcb.27854
[10]	ASAI A, MIYAZAWA T. Dietary curcuminoids prevent high-fat diet-induced lipid accumulation in rat liver and epididymal adipose tissue [J]. The Journal of Nutrition, 2001, 131(11): 2932−2935. doi: 10.1093/jn/131.11.2932
[11]	毛新慧. 山药、姜黄等中药淀粉的理化性质及生理活性的研究[D]. 天津: 天津大学, 2017 MAO X H. Study on the physicochemical properties and biological activities of starch from Dioscorea opposita, Curcuma longa and other Chinese materia Medica[D]. Tianjin: Tianjin University, 2017. (in Chinese)
[12]	DING Y Y, CHENG J J, LIN Q Y, et al. Effects of endogenous proteins and lipids on structural, thermal, rheological, and pasting properties and digestibility of adlay seed (Coix lacryma-jobi L. ) starch [J]. Food Hydrocolloids, 2021, 111: 106254. doi: 10.1016/j.foodhyd.2020.106254
[13]	钟雪瑶, 王少曼, 张彦军, 等. 响应面法优化面包果淀粉的酶法提取工艺 [J]. 食品工业科技, 2020, 41(21):139−144. doi: 10.13386/j.issn1002-0306.2020010004 ZHONG X Y, WANG S M, ZHANG Y J, et al. Optimization of enzymatic extraction technology of starch from breadfruit by response surface methodology [J]. Science and Technology of Food Industry, 2020, 41(21): 139−144.(in Chinese) doi: 10.13386/j.issn1002-0306.2020010004
[14]	SANTANA Á L, ZABOT G L, OSORIO-TOBÓN J F, et al. Starch recovery from turmeric wastes using supercritical technology [J]. Journal of Food Engineering, 2017, 214: 266−276. doi: 10.1016/j.jfoodeng.2017.07.010
[15]	张军, 周佳乐, 王宏亮, 等. 响应面优化超声波提取姜淀粉及其理化性质分析 [J]. 中国食品添加剂, 2022, 33(10):28−37. ZHANG J, ZHOU J L, WANG H L, et al. Ultrasonic extraction of ginger starch by response surface optimization and analysis of physicochemical properties [J]. China Food Additives, 2022, 33(10): 28−37.(in Chinese)
[16]	李敏, 张倩芳, 栗红瑜, 等. 基于不同提取方法对藜麦淀粉性质的比较 [J]. 食品研究与开发, 2022, 43(1):17−24. doi: 10.12161/j.issn.1005-6521.2022.01.003 LI M, ZHANG Q F, LI H Y, et al. Comparison between quinoa starches isolated using different extraction methods [J]. Food Research and Development, 2022, 43(1): 17−24.(in Chinese) doi: 10.12161/j.issn.1005-6521.2022.01.003
[17]	VAN HUNG P, DUYEN T T M, PHI N T L, et al. Fabrication and functional properties of Curcuma starch nanoparticles as affected by different degree of polymerization of debranched Curcuma starch [J]. Starch - Stä rke, 2022, 74(1/2): 2100163.
[18]	KUTTIGOUNDER D, LINGAMALLU J R, BHATTACHARYA S. Turmeric Powder and starch: Selected physical, physicochemical, and microstructural properties [J]. Journal of Food Science, 2011, 76(9): C1284−C1291. doi: 10.1111/j.1750-3841.2011.02403.x
[19]	VAN HUNG P, VO T N D. Structure, physicochemical characteristics, and functional properties of starches isolated from yellow (Curcuma longa) and black (Curcuma caesia) turmeric rhizomes [J]. Starch - Stä rke, 2017, 69(5/6): 1600285.
[20]	国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品中淀粉的测定: GB 5009.9—2016[S]. 北京: 中国标准出版社, 2017.
[21]	傅新征. 薏苡仁淀粉制备与性质研究及改性应用[D]. 福州: 福建农林大学, 2012. FU X Z. Study on preparation, properties, modification and application of the Coix seed starch[D]. Fuzhou: Fujian Agriculture and Forestry University, 2012. (in Chinese)
[22]	祝水兰, 周巾英, 刘光宪, 等. 超声波辅助酸酶法提取碎米抗性淀粉工艺的优化 [J]. 南方农业学报, 2019, 50(8):1814−1821. ZHU S L, ZHOU J Y, LIU G X, et al. Optimization of ultrasonic-assisted acid enzymatic extraction of resistant starch from broken rice [J]. Journal of Southern Agriculture, 2019, 50(8): 1814−1821.(in Chinese)
[23]	初众, 胡美杰, 徐飞, 等. 响应面法优化酶法提取菠萝蜜种子淀粉工艺 [J]. 食品工业科技, 2016, 37(20):189−193,200. CHU Z, HU M J, XU F, et al. Optimization of enzymatic extraction technology condition of starch from jackfruit seed using response surface methodology [J]. Science and Technology of Food Industry, 2016, 37(20): 189−193,200.(in Chinese)
[24]	赵彦巧, 朱志勇, 李建颖, 等. 响应面试验优化中性蛋白酶辅助提取青稞淀粉工艺 [J]. 食品科学, 2016, 37(4):31−36. ZHAO Y Q, ZHU Z Y, LI J Y, et al. Optimization of neutral protease-assisted extraction of highland barley starch [J]. Food Science, 2016, 37(4): 31−36.(in Chinese)
[25]	姜绍通, 殷嘉忆, 王华林, 等. 响应面法优化酶法提取芋头淀粉工艺参数 [J]. 食品科学, 2014, 35(6):24−29. JIANG S T, YIN J Y, WANG H L, et al. Optimization of enzymatic extraction parameters for taro starch [J]. Food Science, 2014, 35(6): 24−29.(in Chinese)
[26]	韩伟, 马婉婉, 骆开荣. 酶法提取技术及其应用进展 [J]. 机电信息, 2010(17):15−18. HAN W, MA W W, LUO K R. Enzymatic extraction technology and its application progress [J]. Mechanical and Electrical Information, 2010(17): 15−18.(in Chinese)
[27]	WANG J Q, LAN T, LEI Y S, et al. Optimization of ultrasonic-assisted enzymatic extraction of kiwi starch and evaluation of its structural, physicochemical, and functional characteristics [J]. Ultrasonics Sonochemistry, 2021, 81: 105866. doi: 10.1016/j.ultsonch.2021.105866
[28]	仪凯, 周瑞宝. 中性蛋白酶水解花生粕的研究 [J]. 中国油脂, 2005, 30(7):71−73. doi: 10.3321/j.issn:1003-7969.2005.07.020 YI K, ZHOU R B. Hydrolysis of peanut meal by neutral protease [J]. China Oils and Fats, 2005, 30(7): 71−73.(in Chinese) doi: 10.3321/j.issn:1003-7969.2005.07.020
[29]	李恬心, 聂沫宇, 吴菲, 等. 响应面优化微波辅助提取佛手山药淀粉的工艺 [J]. 食品工业, 2022, 43(1):61−65. LI T X, NIE M Y, WU F, et al. Microwave assisted extraction of starch from Foshou yam by response surface [J]. The Food Industry, 2022, 43(1): 61−65.(in Chinese)
[30]	孔茂竹, 孔露, 余佳熹, 等. 响应面法优化两步浸泡提取玉米淀粉工艺 [J]. 食品科技, 2019, 44(10):269−275. doi: 10.13684/j.cnki.spkj.2019.10.045 KONG M Z, KONG L, YU J X, et al. Optimization of two-step steeping process for corn starch by response surface methodology [J]. Food Science and Technology, 2019, 44(10): 269−275.(in Chinese) doi: 10.13684/j.cnki.spkj.2019.10.045
[31]	杜洋, 史岩, 陈海华. 响应面法优化高粱米淀粉的中性蛋白酶法提取工艺 [J]. 粮食与油脂, 2015, 28(8):12−16. doi: 10.3969/j.issn.1008-9578.2015.08.004 DU Y, SHI Y, CHEN H H. Optimization of sorghum starch extracting technology with neutral protease hydrolysis by response surface methodology [J]. Cereals & Oils, 2015, 28(8): 12−16.(in Chinese) doi: 10.3969/j.issn.1008-9578.2015.08.004