A RT-PCR Method for Detecting Bovine Rotavirus

LIU Weiwei; LIN Yusheng; ZHANG Long; JIANG Jinxiu; ZHANG Jingpeng; LIU Qinghua; HU Qilin

doi:10.19303/j.issn.1008-0384.2023.04.006

Volume 38 Issue 4

Apr. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 431-435

LIU W W, LIN Y S, ZHANG L, et al. A RT-PCR Method for Detecting Bovine Rotavirus [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：431−435 doi: 10.19303/j.issn.1008-0384.2023.04.006

Citation:

LIU W W, LIN Y S, ZHANG L, et al. A RT-PCR Method for Detecting Bovine Rotavirus [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：431−435 doi: 10.19303/j.issn.1008-0384.2023.04.006

LIU W W, LIN Y S, ZHANG L, et al. A RT-PCR Method for Detecting Bovine Rotavirus [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：431−435 doi: 10.19303/j.issn.1008-0384.2023.04.006

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A RT-PCR Method for Detecting Bovine Rotavirus

doi: 10.19303/j.issn.1008-0384.2023.04.006

LIU Weiwei^{1, 2
,},
LIN Yusheng²,
ZHANG Long^{1, 2},
JIANG Jinxiu²,
ZHANG Jingpeng²,
LIU Qinghua^{1
,
,},
HU Qilin^{2
,
,}

1.
College of Animal Science(College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

Received Date: 2022-06-09
Accepted Date: 2022-06-09
Rev Recd Date: 2022-10-22

Available Online: 2023-04-14

Publish Date: 2023-04-28

Abstract

Abstract

Objective A method for bovine rotavirus (BRV) detection was developed to determine the current epidemiological status in Fujian province. Method A pair of specific primers was designed based on the BRV genome from GenBank using Oligo7.0. Reaction conditions of the RT-PCR method were optimized for the detection. Specificity, sensitivity, and repeatability of the assay were verified on 22 clinical samples. Result The newly developed method amplified only the specific fragment of BRV, not any of other common pathogens of livestock and poultry. A sensitivity of 6.86×10⁵ copies·μL⁻¹ was achieved. And, on the 22 clinical samples, 12 were tested positive at a rate of 54.55%. Conclusion The established RT-PCR method for BRV detection displayed high specificity, sensitivity and repeatability, which could be applied for clinical diagnosis and epidemiological investigation.
- Bovine rotavirus,
- detection method,
- RT-PCR

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References(16)

References

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