Anti-TMV Activity of Chemicals in Leaves and Branches of <i>Ailanthus altissima</i>

WU Jiangmei; ZUO Anjian; ZOU Zhengbiao; LIU Yang; ZHANG Wei; FU Tao; YAN Xiaohui; HU Shijun

doi:10.19303/j.issn.1008-0384.2023.04.009

Volume 38 Issue 4

Apr. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 454-460

WU J M, ZUO A J, ZOU Z B, et al. Anti-TMV Activity of Chemicals in Leaves and Branches of Ailanthus altissima [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：454−460 doi: 10.19303/j.issn.1008-0384.2023.04.009

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WU J M, ZUO A J, ZOU Z B, et al. Anti-TMV Activity of Chemicals in Leaves and Branches of Ailanthus altissima [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：454−460 doi: 10.19303/j.issn.1008-0384.2023.04.009

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Anti-TMV Activity of Chemicals in Leaves and Branches of Ailanthus altissima

doi: 10.19303/j.issn.1008-0384.2023.04.009

1.
Key Laboratory of Forest Disaster Warning and Control in Yunnan Province/College of Biodiversity Conservation, Southwest Forestry University, Kunming, Yunnan　650233, China
2.
College of Forestry，Southwest Forestry University, Kunming, Yunnan　650233, China

Received Date: 2022-10-17
Rev Recd Date: 2023-02-25

Available Online: 2023-05-09

Publish Date: 2023-04-28

Abstract

Abstract

Objective Chemical composition and anti-tobacco mosaic virus (TMV) activity of Ailanthus altissima were studied to pave the way for developing an effective viral inhibitor. Methods Substrates in the n-butanol extract of leaves and branches of A.altissima plants were separated using silica gel, Sephadex LH-20, and MCI column chromatography.Chemical structures of the substrates were determined based on NMR and MS data, and anti-TMV activity of the compounds examined using the half-leaf method. Results The 17 compounds isolated from the n-butanol extract were identified to be: (1) kaempferol, (2) (2S)-3-o-octadeca-9Z, 12Z, 15Z-trienoylgycery-O-β-D-galactopyranoside, (3) hexacosane, (4) 6,9,12-octadecatrienoic acid, (5) eichlerianic acid, (6) colocasinol A, (7) caffeic acid eicosanyl ester, (8) acernikol, (9) (-)-Sakuyayesinol (10), (14S,17S, 20S, 24R)-20,24,25-trihydroxy-14,17-cylomalabarican-3-one, (11) 4-(3-butoxy-1-hydroxy-2-methoxypropyl) benzene-1, 2-diol, (12) pinnata, (13) trans-syringin, (14) gingerglycolipid A, (15) gingerglycolipid B, (16) benzyl 2-o-β-apiofuranosyl-(1→2)-β-D-glucopyranosyl-2,6-dihydroxy-benzoate, and (17) picrorhizoside C.Among them, Compounds 2, 14, and 15 were isolated from the plant for the first time.And the lignans, phenylpropanoids, or galactose lipids, such as Compounds 6, 8, 13, and 14 at concentration of 50 μg·mL⁻¹ displayed a TMV inactivation rate greater than 50%, which was similar to that of the positive control, ningnamycin.For Compounds 6, 13, and 14, the rates were even higher than that of ningnamycin. Conclusion All 17 substances isolated from the leaves and branches of A.altissima exhibited varying degrees of inhibitory effect on TMV.The efficacy was more significant as shown by certain Lignans, phenylpropanoids, and galactose lipids.
- Ailanthus altissima,
- chemical composition,
- isolation and identification,
- half-leaf method,
- anti-TMV activity

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References(33)

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