Identification and Analysis of Tn5 Transposed Insert Sites in Avirulent Mutants of Ralstonia solanacearum

Mutants of Ralstonia solanacearum strain FJAT-91 using Tn5 transposon mutagenesis were developed in this paper,which were named FJAT-t582 and FJAT-t583.Colony morphology and attenuation indexes showed that both of the mutants FJAT-t582 and FJAT-t583 were avirulent and their attenuation indexes were over 0.8.A 681 bp fragment of the kanamycin resistance gene was amplified from the two genome DNA of mutants.The insertion sites were located in putative diguanylate phosphodiesterase and type Ⅲ effector protein gene respectively.And they may cause the pathogenicity reduction of the R.solanacearum.