Construction and Verification of Trans-linked Double T-DNA Plant Expression Vector

We constructed two trans-linked double T-DNA plant expression vectors by modification of the structure of the double T-DNA,p1300-DMAR-LF-GCMSAGS and p1300-DMAR-LF-GLCMSAGS.They were verified by transgenic rice plants.The results indicated that the selectable marker-free transgenic plants could be obtained.The frequencies of unlinked co-transformants transformed by the trans-linked double T-DNA plant expression vectors were increased from 47.37% and 45.83% to 55.81% and 51.28% compared to those transformed by the cis-linked double T-DNA plant expression vectors,indicated that the efficiency on the selectable marker-free transgenic plant was improved.