Cloning and Sequence Analysis on Gene of Chlorophyll a/b-binding Protein from Narcissustazetta var. chinensis

From a cDNA fragment of the suppression subtractive hybridization of Narcissus tazetta var.chinensis induced by paclobutrazol,the gene of the light-harvesting chlorophyll a/b-binding protein,Ntcab1,was isolated and cloned by RACE and RT-PCR.The full length of Ntcab1cDNA was determined to be 1 012bp with a 798bp ORF,which encoded a protein of 265 amino acids with a predicted molecular weight of 28.155kD and a theoretical isoelectric point of 5.48.The phylogenetic analysis showed that Ntcab1could be clustered together with LHCb1.1 from Arabidopsis thaliana,and had a close relationship with those in Ophiopogon japonicus(ACD64978.1)and Lycoris radiata(ADC35579.1).The semi-quantitative RT-PCR revealed that the expression of Ntcab1was higher during sprouting and early flowering after it was sprayed with paclobutrazol than water.