Optimization of Tissue Culture for Propagating Clustered Shoots of Phalaenopsis

Adventitious shoots induced from the peduncle auxiliary buds of Phalaenopsis‘Lauluns' were used for the study.The physical segmentation, placement mode and concentration of inorganic salt and organic additives all showed significant effects on the proliferation of Phalaenopsis with clustered shoots.Among the treatments, the greatest average number of inductive buds occurred when a bud was excised at the growth point and then grafted horizontally (PSP-3) .However, the bud's death rate was as high as 23.3%.In contrast, when the double buds were used, and followed by the horizontal grafting (PSP-6) , the budding was high, but the death rate was low.The inorganic salt concentration between 1/3MS to 1/2MS was found to be suitable for the propagating with strong seedling growth.Addition of coconut juice at 15% (v/v) level was desirable for the Phalaenopsis cluster buds proliferation, while 150g·L-1 potato juice or banana puree could be applied as low cost organic additives in the culture medium.