Rapid Detection of Duck Tembusu Virus Antibody by Latex Agglutination Test

Duck tembusu viruses were cultivated and concentrated by ultracentrifugation and density-gradient centrifugation to determine the best suitable conditions for sensitized latex and prepare antigen for the latex agglutination test (LAT) by titration.The agglutination particles could be observed clearly by naked eyes after antigen reaction with positive duck tembusu virus serum.The sensitized antigen had no cross-reaction with avian influenza virus, avian paramyxovirus type 1, duck hepatitis virus type 1, egg drop syndrome virus, and duck reovirus.Of 80duck serums, 58and 68 were detected to be positive by the established LAT and ELISA, respectively;the positive rates for both were above 85.3% with no statistical difference.The results revealed that the established LAT was simple, quick and specific for the rapid diagnose and survey of duck tembusu virus antibody.