Cloning and Sequence Analysis of Chloroplast ATP Synthase Gene NtATPFfrom Narcissus tazetta var.Chinensis

Based on a fragment cDNA from suppression subtractive hybridization (SSH) of Narcissus tazetta var.chinensis induced by paclobutrazol, an ATP Synthase CF0 subunit gene NtATPF was separated and cloned by RACE and RT-PCR.The full length of NtATPFcDNA was 905 bp with a 555 bp ORF, which encoded a protein of184 amino acids and had a transmembrance domain (27-49) with a predicted molecular mass of 20.809 5kD and theoretical isoelectric point of 5.69.Phylogenetic analysis showed that NtATPF had close relationship to ATP Synthase CF0 subunit in Allium fistulosum (AEX93661.1) .