Herpesvirus Isolation and Identification of the Cultured Eel with ‘Red Liver Disease'

394bp fragment was amplified and cloned from genomic DNA of the diseased A.Rostrataand A.bicolor bicolor.The nucleotide homology was 100% with DNA polymerase of 6eels herpesvirus(HQ992949,GU233800,FJ940765,EU349272,EU349271,AF333066)and 99% of 2eels herpesvirus(GU205167,AF363783).The suspension of gill,liver and kidney from diseased A.bicolor bicolor and diseased A.Rostrata which filtered through a filter membrane to get rid of bacteria was used to infected EPC cells,CPE appeared after 7d.Now we have cultivated 14 generations fromA.bicolor bicolor and 5generations fromA.Rostrata.