Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)

A 1309 bp full length cDNA sequence of PsACO1 gene were isolated from Furong plum(Prunus salicina Lindl.)through RT-PCR and RACE technology.The isolated gene contains a 957 bp open reading frame and encodes a protein of 319 amino acids with isoelectric point of 5.28 and molecular weight of 36.24 kD.PsACO1protein contains characteristic domains of ACO proteins.The phylogenetic analysis results showed that PsACO1 has close corelation with ACO protein fromPrunus persica,Prunus mume,Eriobotryajaponica,Malus domestica and Prunus domestica.