• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊
FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she, LUO Yuan-hua. Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Oncidium[J]. Fujian Journal of Agricultural Sciences, 2015, 30(7): 697-700. DOI: 10.19303/j.issn.1008-0384.2015.07.015
Citation: FAN Rong-hui, HUANG Min-ling, ZHONG Huai-qin, WU Jian-she, LUO Yuan-hua. Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Oncidium[J]. Fujian Journal of Agricultural Sciences, 2015, 30(7): 697-700. DOI: 10.19303/j.issn.1008-0384.2015.07.015

Multiplex RT-PCR for Simultaneous Detection of Three Viruses in Oncidium

  • By using specific primers designed on the basis of conserved sequences of coat protein gene,a multiplex PCR protocol for the detection of 3commonly found disease-causing viruses,i.e.,Cymbidium mosaic(CyMV),Odontoglossum ringspot(ORSV)and bean yellow mosaic(BYMV),in Oncidium was developed.Three specific fragments were simultaneously amplified in a single PCR reaction system.Their molecular weights were determined to be 571 bp for CyMV,325 bp for ORSV,and 212 bp for BYMV.Sequence analysis indicated that the 3viruses were at least 98% homogenous with their respective reference viruses.The sensitivity test on this newly developed methodology showed that it was capable of accurately detecting the 3viruses simultaneously in a bulbous iris tissue at the level of 10-2 mg.
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