Cloning and Bioinformatic Analysis of HOS1 Promoter from the 'Tianbao' Banana (Musa acuminata. AAA group)

In this experiment, HOS1 promoter was cloned successfully from the 'Tianbao' banana, and was further bioinformatically predicted. The analysis results showed that the sequence similarity of HOS1 promoter was 92.43% between the Tianbao banana and the wild banana in Malaysia, and the Tianbao banana promoter contained 35 kinds of cis-elements, including a variety of types of phytohormone response cis-elements and cis-elements concerning abiotic stress, which suggested that there be a close relationship with cold stress response in Tianbao banana. Tianbao banana HOS1 promoter contained two CpG islands, which suggested it should also have a close relationship between cold stress response and methylation in Tianbao banana.