Research of Fonctuonal Region Involved in the Self-interaction of Rice Stripe Virus Coat Protein

Coat protein of Rice stripe virus plays important roles in several biological processes, such as viral transcription and replication. Interactions among viral proteins are essential for viral infection activity on host and insect vectors. In the present study, yeast two-hybrid system was used to detect the functional region inolving in the self-interaction of the RSV coat protein (CP). It was revealed that N-and C-terminal 81 amino acid residues played an essential role in self-interaction of RSV CP. Our results will be contributed to better understanding not only for functions of CP on viral stabilization and replication in insect vectors or host plants, but also for the relationship among virus, insect vectors and host plants.