Establishment and Monitoring Analysis of Fluorescence RT-PCR for Detection of Bovine Viral Diarrhea Virus in Swine

The study was aimed at developing a quantitative real-time fluorescence reverse transcriptase PCR(qRT-PCR) assay according to the conserved gene sequence of BVDV 5'-UTR for detection of bovine viral diarrhea virus(BVDV) obtained from 359 slaughtered swine serum samples of slaughterhouses in Fuzhou Prefecture. The results showed that the BVDV positive rate of slaughtered swines was 14.5%(52 positive samples out of 359 ones). Sequence analysis on 5'-UTR fragments of 13 BVDV positive samples showed that 5'-UTR fragments of all tested samples were closely related to VEDEVAC evolution lineages, all of which were genotype I. It was indicated that the specific qRT-PCR method established based on BVDV was suitable for detection of BVDV in swines. And the BVDV infection was common in slaughtered swines of Fuzhou Prefecture.