Induction Plant Regeneration with Stem Segments of Anoectochilus roxburghii (wall.) lindl. Huaye

Stem segments of Anoectochilus roxburghii (wall.) lindl. Huaye were used as the explants to induce bud clumps. Varied medium (i.e., MS, B₅ or improved MS) and phytohormone (i.e., TDZ, 6-BA, KT or IBA) were applied for the tissue regeneration experiment of an orthogonal design. It was found the major factors affecting the bud multiplication to be basal medium, KT, 6-BA and TDZ. The optimal multiplication was achieved by using the improved MS+6-BA with additions of 2.0 mg·L^-1+KT 0.5 mg·L^-1+NAA 0.1 mg·L^-1+sugar 30.0 g·L^-1+agar powder 3.0 g·L^-1, and carrageenan 3.6 g·L^-1. An averaged propagation coefficient of 4.58 in 60 days was obtained. For rooting, the improved MS+6-BA with added 0.3 mg ·L^-1+AC 0.5 g·L^-1+sugar 20.0 g·L^-1+agar powder 3.6 g·L^-1 and carrageenan 3.6 g·L^-1, rendered a 100.0% rate in 60 days.