Tissue Culture Regeneration of Fast-growing Taiwan Poplar

A regeneration protocol was established using the stems and leaves of fast-growing Taiwan Poplar 3 as explants. The selected disinfection time for the stems was 10 min in a 0.15% HgCl₂ solution to result in an infection rate of 23.3% and a budding rate of 75.3%. The optimized medium for generating adventitious buds was MS+BA 0.3 mg·L^-1+NAA 0.1 mg·L^-1 to reach an average differentiation rate of 77.88%; that for the leaf differentiation, MS+BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1+KT 0.2 mg·L^-1+adenine 40 mg·L^-1 with a differentiation rate of 87.7%; that for the proliferation, MS+BA 0.5 mg·L^-1+KT 0.3 mg·L^-1+NAA 0.2 mg·L^-1; and that for rooting, 1/2MS+NAA 0.2 mg·L^-1+CCC 0.5 mg·L^-1 with a 100% rooting rate.